Lotareva O V
Genetika. 1978 Nov;14(11):2046-8.
The UV-irradiation of Bacillus subtilis Mu5u8u16 (met5 leu8 purA) induces with relatively high frequency the revertants to adenine independence (Ade+) which form the rapidly growing morphologically uniform colonies on the solid selective medium. The genetic analysis of a portion of UV-induced Ade+ revertants (crosses in transformation system) has cleared up that their DNA does not contain the original mutation ade16. This means that they arise as the result of "true" reversions. This reversion in purA gene can serve as a good model for the study of UV-induced mutagenesis in a proximal structural locus of Bac. subtilis chromosome.
枯草芽孢杆菌Mu5u8u16（met5 leu8 purA）经紫外线照射后，能以相对较高的频率诱导出腺嘌呤非依赖型（Ade+）回复突变体，这些突变体在固体选择培养基上形成生长迅速、形态均一的菌落。对部分紫外线诱导的Ade+回复突变体进行遗传分析（转化系统中的杂交实验）后发现，它们的DNA中不包含原始的ade16突变。这意味着它们是“真正”回复突变的结果。purA基因中的这种回复突变可作为研究枯草芽孢杆菌染色体近端结构基因座中紫外线诱导诱变的良好模型。
