Frenkel' L A
Vopr Med Khim. 1976 May-Jun;22(3):410-3.
A modified method is developed for estimation of citric acid in bone tissue. Citrate was isolated from acid bone hydrolysate without a preliminary deproteinization; then it was oxidized and converted into pentabromaceton by treatment of citric acid with bromine. The content of pentabromaceton was estimated by a photometric method. The method was highly sensitive and reproductible.
开发了一种改良方法用于估算骨组织中的柠檬酸。从酸性骨水解物中分离出柠檬酸盐,无需预先进行脱蛋白处理;然后将其氧化,通过用溴处理柠檬酸将其转化为五溴丙酮。通过光度法估算五溴丙酮的含量。该方法具有高度敏感性和可重复性。