在细胞培养基中用激素替代血清。

The replacement of serum by hormones in cell culture media.

作者信息

Sato G, Hayashi I

出版信息

Arch Biol Med Exp. 1976 Dec;10(1-3):120-1.

Abstract

The replacement of serum by hormones in cell culture media. (Reemplazo del suero por hormonas en el medio de cultivo de células). Arch. Biol. Med. Exper. 10: 120-121, 1976. The serum used in cell culture media can be replaced by a mixture of hormones and some accesory blood factors. The pituitary cell line GH3 can be grown in a medium in which serum is replaced by triiodothyronine, transferrin, parathormone, tyrotrophin releasing hormone and somatomedins. Hela and BHK cell strains can also be grown in serum free medium supplemented with hormones. Each cell type appears to have different hormonal requirements yet it may found that some hormones are required for most cell types.

摘要

细胞培养基中血清被激素替代。（细胞培养基中血清被激素替代。）《实验生物学与医学档案》10: 120 - 121，1976年。细胞培养基中使用的血清可被激素混合物和一些辅助血液因子替代。垂体细胞系GH3可在一种培养基中生长，该培养基中血清被三碘甲状腺原氨酸、转铁蛋白、甲状旁腺激素、促甲状腺激素释放激素和生长调节素替代。Hela和BHK细胞株也可在补充了激素的无血清培养基中生长。每种细胞类型似乎有不同的激素需求，但可能会发现某些激素是大多数细胞类型所必需的。