Thomas D B, Smith C M, Sumpster J M
J Anat. 1976 Sep;122(Pt 1):15-22.
The proliferation of transplanted murine haematopoietic cells, derived from the femoral bone marrow of young adults and from the liver on the sixteenth day of gestation, has been compared under standardized conditions. Suspensions of bone marrow cells contained 9-6+/-0-9 CFU per 10(5) cells (sm) and suspensions of fetal liver cells contained 3-7+/-0-5 CFU per 10(5) cells (Sl).Sl/Sm = 0-4. The mean diameter of colonies established by cells derived from bone marrow was 1-3+/-0-3 mm (established mean volume, Vm = 1-2 mm3) and the mean diameter of colonies established by cells derived from fetal liver was 1-7+/-0-1 mm (estimated mean volume, Vl ;-6 mm3). Vl/Vm ;-2. The relationship between the weight of the spleen and the number of bone marrow cells injected into lethally irradiated recipients 10 days previously has been confirmed, and a similar relationship between the weight of the spleen and the number of fetal liver cells injected has been demonstrated. An arbitrarily defined difference of 50 mg between the weight of the spleen in untreated irradiated controls and the weight of the spleen in irradiated recipients of haematopoietic cells has been observed 10 days after the administration of 2-2 x 10(6) bone marrow cells (Dm) or 3-0 x 10(6) fetal liver cells (Dl). Dm/Dl = 0-7. The calculated values of Dm/Dl (relative proliferative capacity), Sl/Sm (relative stem cell content) and Vl/Vm (relative clone size) are in good agreement with the values estimated using the equation Dm/Dl = Sl/Sm x Vl/Vm.
在标准化条件下，对源自年轻成年小鼠股骨骨髓和妊娠第16天肝脏的移植鼠造血细胞的增殖情况进行了比较。每10⁵个骨髓细胞悬液中含有9 - 6±0 - 9个集落形成单位（CFU）（sm），每10⁵个胎肝细胞悬液中含有3 - 7±0 - 5个CFU（Sl）。Sl/Sm = 0 - 4。由骨髓来源的细胞形成的集落平均直径为1 - 3±0 - 3毫米（确定的平均体积，Vm = 1 - 2立方毫米），由胎肝细胞形成的集落平均直径为1 - 7±0 - 1毫米（估计平均体积，Vl = - 6立方毫米）。Vl/Vm = - 2。已经证实了脾脏重量与10天前注射到致死性照射受体体内的骨髓细胞数量之间的关系，并且也证明了脾脏重量与注射的胎肝细胞数量之间存在类似关系。在给予2 - 2×10⁶个骨髓细胞（Dm）或3 - 0×10⁶个胎肝细胞（Dl）10天后，观察到未处理的照射对照小鼠脾脏重量与造血细胞照射受体小鼠脾脏重量之间任意定义的50毫克差异。Dm/Dl = 0 - 7。Dm/Dl（相对增殖能力）、Sl/Sm（相对干细胞含量）和Vl/Vm（相对克隆大小）的计算值与使用公式Dm/Dl = Sl/Sm×Vl/Vm估计的值高度一致。
