Kiselev G V, Pavlinova L I
Vopr Med Khim. 1976 Nov-Dec;22(6):848-51.
Total phosphorus was estimated after mineralization of a sample, containing organic phosphoesters and proteins; a method is based on the formation of phosphomolybdenovanadium complex at acid pH. The analyses for inorganic phosphorus were carried our together with estimation if some labile phosphoesters (glucose-I-phosphate, ADP, ATP), which was possible due to low acidity of the medium (0.24 N HC1O4), small concentrations of reagents (3.2 mg/ml of molybdate, 0.08 mg/ml vanadate), addition of sodium citrate to neutralize the excess of molybdenovanadium acid and extraction of the colored complex with butyl or isobutyl alcohol. Optical density was measured at two wave lengths (340 nm and 390 nm) which enabled to estimate from 0.5 to 80 microng of phosphrorus in a sample.
在对含有有机磷酸酯和蛋白质的样品进行矿化后测定总磷；该方法基于在酸性pH条件下形成磷钼钒络合物。无机磷的分析与一些不稳定磷酸酯（葡萄糖-1-磷酸、ADP、ATP）的测定同时进行，这是由于介质的低酸度（0.24N高氯酸）、试剂的低浓度（钼酸盐3.2mg/ml、钒酸盐0.08mg/ml）、添加柠檬酸钠以中和过量的磷钼钒酸以及用丁醇或异丁醇萃取有色络合物而得以实现的。在两个波长（340nm和390nm）下测量光密度，这使得能够估算样品中0.5至80微克的磷。
