Keenan K, Erlich E, Donnelly K H, Basel M B, Hutner S H, Kassoff R, Crawford S A
J Protozool. 1978 Aug;25(3 Pt 2):385-7. doi: 10.1111/j.1550-7408.1978.tb03911.x.
Autoclavable, natural particulate media simplify axenic cultivation of tetrahymenid ciliates and presumably favor selection for phagotrophy. Viability is at least 2 months at room temperature (24-26 C) for the lipid-sensitive tetrahymenids Tetrahymena setosa, T. corlissi, T. paravorax, T. limacis, and T. patula, also for T. rostrata and (at 12 C), for strains of the T. pyriformis complex and Glaucoma chattoni. A typical medium consists of crude soy "lecithin" + skim milk powder + Saccharomyces cerevisiae cells. Other useful particules readily available commercially are: whole liver powder, cells of Micrococcus lysodeikticus and Escherichia coli, and powdered residue of liver which had been extracted with 70% ethanol ("liver No. 2). Preliminary experiments indicate that some of these media are suitable for the maintenance of Paramecium octaurelia stock 299S and Colpidium campylum. Such mixtures may serve as points of departure for devising media for more fastidious phagotrophs.
可高压灭菌的天然颗粒培养基简化了四膜虫纤毛虫的无菌培养，并且可能有利于选择吞噬营养。对于对脂质敏感的四膜虫，如刚毛四膜虫、科氏四膜虫、副口四膜虫、蛞蝓四膜虫和小尾四膜虫，在室温（24 - 26℃）下其活力至少可保持2个月，对喙状四膜虫以及梨形四膜虫复合体的菌株和恰氏眼虫（在12℃）也是如此。一种典型的培养基由粗制大豆“卵磷脂” + 脱脂奶粉 + 酿酒酵母细胞组成。其他易于商业获取的有用颗粒包括：全肝粉、溶壁微球菌和大肠杆菌的细胞，以及用70%乙醇提取过的肝脏粉末残渣（“2号肝脏”）。初步实验表明，其中一些培养基适用于维持八尾草履虫299S品系和栉毛虫的培养。这样的混合物可为设计更挑剔的吞噬营养生物的培养基提供出发点。
