Smirnova O V, Smirnov A N, Rozen V B
Probl Endokrinol (Mosk). 1976 Jul-Aug;22(4):87-92.
Some properties of the macromolecules, specifically binding estradiol (E2) of salt chromatin extracts of the nuclei of the kidneys, liver, prostate, testes and the uterus of rats were studied. Examination of competition of various unlabeled hormonal preparations with 3H-E2 for the binding sites demonstrated the E2-binding macromolecules of the nuclei of all the organs under study to have a high hormonal stereo-specificity. The E2-binding macromolecules of the nuclear extracts of the kidney, liver and the uterus were sensitive to the action of pronase. In sedimentation with ammonium sulfate of proteins from the 0.3 M NaCl extracts of the nuclei of the kidneys and liver, but not of the uterus, the supernatent fraction of the salt-treated extracts showed an additional (in comparison with control) specifically E2-binding macromolecule activity, sensitive to the action of pronase. An analogous effect of the appearance of additional binding activity could be obtained in heating of the nuclear extracts of the kidney and the liver at 60--100 degress C for 5 minutes.
研究了大鼠肾脏、肝脏、前列腺、睾丸和子宫细胞核盐染色质提取物中大分子的一些特性，特别是结合雌二醇（E2）的特性。用各种未标记的激素制剂与3H-E2竞争结合位点的实验表明，所研究的所有器官细胞核中的E2结合大分子具有高度的激素立体特异性。肾脏、肝脏和子宫细胞核提取物中的E2结合大分子对链霉蛋白酶的作用敏感。在用硫酸铵沉淀肾脏和肝脏细胞核0.3M NaCl提取物中的蛋白质时（子宫提取物则不然），盐处理提取物的上清部分显示出额外的（与对照相比）特异性E2结合大分子活性，对链霉蛋白酶的作用敏感。在60-100摄氏度下将肾脏和肝脏的核提取物加热5分钟，也可获得类似的额外结合活性出现的效果。
