[相分配法在疏水膜蛋白分级分离中的应用]
[Application of phase partition method for the fractionation of hydrophobic membrane proteins].
作者信息
Lukoianova M A, Petukhova N M
出版信息
Biokhimiia. 1976 Oct;41(10):1810-8.
PMID:1030632
Abstract
Phase partition method in a two-phase polyethylene glycol-dextrane system has been applied to fractionation in Triton X-100 of hydrophobic membrane proteins from Micrococcus lysodeikticus. This method allowed to separate the cytochrome b556 from other cytochromes. Spectral and gel electrophoretic patterns of isolated cytochrome are given.
摘要
两相聚乙二醇-葡聚糖系统中的相分配法已应用于从溶壁微球菌中分离疏水膜蛋白的Triton X-100分级分离。该方法能够将细胞色素b556与其他细胞色素分离。给出了分离出的细胞色素的光谱和凝胶电泳图谱。
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