Braund K G, Ghosh P, Taylor T K, Larsen L H
Res Vet Sci. 1976 Nov;21(3):314-7.
Through the use of a critical electrolyte concentration staining technique, the glycosaminoglycans (GAGs) in the nucleus pulposus region of the canine intervertebral disc were arbitrarily identified as "hyaluronic acid", chondroitin sulphate and keratan sulphate. Approximate estimates of GAG concentration could be qualitatively deduced by determining the appropriate GAG "ALCIANOPHILIC INDEX". This index was considered to be an effective qualitative adjunct to chemical quantitation of GAGs in the intervertebral disc.
通过使用一种关键电解质浓度染色技术，犬椎间盘髓核区域的糖胺聚糖（GAGs）被随意鉴定为“透明质酸”、硫酸软骨素和硫酸角质素。通过测定适当的GAG“阿利新蓝嗜染指数”，可以定性推断GAG浓度的大致估计值。该指数被认为是椎间盘GAGs化学定量的一种有效的定性辅助手段。
