Loginova L G, Guzhova E P, Ismanlova D Iu, Burdenko L G
Prikl Biokhim Mikrobiol. 1978 Jul-Aug;14(4):485-93.
The fungus Aspergillus terreus 17P--producer of cellulolytic enzymes--was cultivated in the Biotec 10 l fermenter on the medium containing minced and heated (at 200 degrees) wheat straw aerated with a different rate. At the mixing rate of 350 rpm and aeration rate of 0.7 r/rpm on the fourth day the culture liquid was obtained whose filtrate contained an active complex of cellulolytic enzymes and xylanase: CI--3.4; APB--1.1, Cx--35.7, cellobiase--0.23, xylanase--73.8 units/ml. The fractionation of the culture liquid filtrate with ammonium sulphate showed that the fraction precipitated at an interval of saturation of 0.3--0.7 contained the largest portion of cellulolytic enzymes and xylanase. The isolated enzymic preparations had a cellulolytic and xylanase activity and contained lipase, pectinase, laminarinase. They also contained low quantities of amylase, protease, beta-1,4- and beta-1,6-glucanase. Enzymic hylrolysis by the Asp. terreus 17P preparation of straw yielded glucose and xylose, of cotton, Na-KMC, cellobiose--glucose, Xylane hydrolyzate contained xylose and arabinose.
将纤维素分解酶的产生菌——土曲霉17P接种于装有切碎并加热（200℃）的小麦秸秆的10升Biotec发酵罐中，采用不同的通气速率进行培养。在350转/分钟的搅拌速率和0.7升/转/分钟的通气速率下，于第4天获得培养液，其滤液中含有纤维素分解酶和木聚糖酶的活性复合物：内切葡聚糖酶（CI）——3.4；滤纸糖酶（APB）——1.1，外切葡聚糖酶（Cx）——35.7，纤维二糖酶——0.23，木聚糖酶——73.8单位/毫升。用硫酸铵对培养液滤液进行分级分离表明，在饱和度为0.3至0.7的区间沉淀的级分中含有最大比例的纤维素分解酶和木聚糖酶。分离得到的酶制剂具有纤维素分解和木聚糖酶活性，且含有脂肪酶、果胶酶、海带多糖酶。它们还含有少量的淀粉酶、蛋白酶、β-1,4-和β-1,6-葡聚糖酶。土曲霉17P制剂对秸秆进行酶解产生葡萄糖和木糖，对棉花、钠-羧甲基纤维素进行酶解产生纤维二糖——葡萄糖，木聚糖水解产物含有木糖和阿拉伯糖。
