Sato-Nara K, Yuhashi K I, Higashi K, Hosoya K, Kubota M, Ezura H
Plant Biotechnology Institute, Ibaraki Agricultural Center, Iwama, Nishi-ibaraki 319-0292, Japan.
Plant Physiol. 1999 May;120(1):321-30. doi: 10.1104/pp.120.1.321.
We isolated two muskmelon (Cucumis melo) cDNA homologs of the Arabidopsis ethylene receptor genes ETR1 and ERS1 and designated them Cm-ETR1 (C. melo ETR1; accession no. AF054806) and Cm-ERS1 (C. melo ERS1; accession no. AF037368), respectively. Northern analysis revealed that the level of Cm-ERS1 mRNA in the pericarp increased in parallel with the increase in fruit size and then markedly decreased at the end of enlargement. In fully enlarged fruit the level of Cm-ERS1 mRNA was low in all tissues, whereas that of Cm-ETR1 mRNA was very high in the seeds and placenta. During ripening Cm-ERS1 mRNA increased slightly in the pericarp of fruit before the marked increase of Cm-ETR1 mRNA paralleled climacteric ethylene production. These results indicate that both Cm-ETR1 and Cm-ERS1 play specific roles not only in ripening but also in the early development of melon fruit and that they have distinct roles in particular fruit tissues at particular developmental stages.
我们从甜瓜（Cucumis melo）中分离出拟南芥乙烯受体基因ETR1和ERS1的两个cDNA同源物，分别命名为Cm-ETR1（甜瓜ETR1；登录号AF054806）和Cm-ERS1（甜瓜ERS1；登录号AF037368）。Northern分析表明，果皮中Cm-ERS1 mRNA的水平随着果实大小的增加而平行升高，然后在膨大末期显著下降。在完全膨大的果实中，所有组织中Cm-ERS1 mRNA的水平都很低，而Cm-ETR1 mRNA在种子和胎座中非常高。在成熟过程中，在果实果皮中，Cm-ERS1 mRNA在Cm-ETR1 mRNA显著增加并伴随呼吸跃变型乙烯产生之前略有增加。这些结果表明，Cm-ETR1和Cm-ERS1不仅在甜瓜果实成熟过程中，而且在其早期发育过程中都发挥着特定作用，并且它们在特定发育阶段的特定果实组织中具有不同的作用。
