Lee J H, Van Montagu M, Verbruggen N
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5873-7. doi: 10.1073/pnas.96.10.5873.
Osmotic stress (drought, salt stress) is a major limiting factor for crop productivity in the world. Because cellular responses to osmotic stress are thought to be conserved in eukaryotes and because yeast is much more amenable than plants to genetic research, a functional strategy has been performed to identify limiting steps in osmotolerance of plants based on the complementation of yeast with a plant library. A new plant cDNA that encodes a functional homologue of the yeast Dbf2 kinase enhances salt, drought, cold, and heat tolerance upon overexpression in yeast as well as in transgenic plant cells.
渗透胁迫(干旱、盐胁迫)是全球作物生产力的主要限制因素。由于真核生物中细胞对渗透胁迫的反应被认为是保守的,并且酵母比植物更适合进行遗传研究,因此已实施一种功能策略,基于用植物文库对酵母进行互补来确定植物渗透耐受性的限制步骤。一种编码酵母Dbf2激酶功能同源物的新植物cDNA,在酵母以及转基因植物细胞中过表达时可增强对盐、干旱、寒冷和热的耐受性。