Krasnow N
Recent Adv Stud Cardiac Struct Metab. 1976;11:297-304.
The interaction of gadolinium, a lanthanide with calcium-blocking action, with isolated muscle microsomes has been studied. Two classes of binding sites were present, 80 and in excess of 300 nmol/mg in number, respectively. Divalent cation, including Ca, Zn, and Cd, blocked the higher affinity site. Antibiotic ionophores X537A and A23187 enhanced binding, with positive cooperativity. ATP enhanced binding at low concentration (10-20 muM) of nucleotide, and without ATP-hydrolysis. The data suggest a dissociation in the intact membrane between the binding and ATP-hydrolytic portion of the transport site.
已对具有钙阻断作用的镧系元素钆与分离的肌肉微粒体之间的相互作用进行了研究。存在两类结合位点，数量分别为80和超过300 nmol/mg。二价阳离子，包括钙、锌和镉，阻断了高亲和力位点。抗生素离子载体X537A和A23187增强了结合，并具有正协同性。ATP在低浓度（10 - 20 μM）核苷酸时增强结合，且无需ATP水解。数据表明在完整膜中转运位点的结合部分和ATP水解部分之间存在解离。
