Al-Janabi N, Olson H E, Ammerante T, Ross D N
Recent Adv Stud Cardiac Struct Metab. 1976;12:549-56.
Changes in metabolic activity of removed heart valve allografts have been measured. The fresh heart valves were sterilized and stored in antibiotic solution before implantation in patients. Viability was determined before insertion and after removal from patients by two methods: 1) tissue culture, and 2) autoradiography, using tritiated thymidine. The length of storage in the Hank's antibiotic or nutrient-antibiotic medium before insertion did not seem to influence the final metabolic activity nor the structural integrity of the allografts when they were removed. Results from the present study show that the most severe degenerative changes occur in valves stored in Hank's solution and then implanted in the mitral position. The viability percentage declined progressively during the time that a valve treated in this manner was functioning in a patient.
已对移除的同种异体心脏瓣膜的代谢活性变化进行了测量。新鲜心脏瓣膜在植入患者体内之前进行了灭菌处理，并保存在抗生素溶液中。通过两种方法在植入前和从患者体内移除后测定其活力：1）组织培养，以及2）使用氚标记胸腺嘧啶核苷的放射自显影法。植入前在汉克氏抗生素或营养-抗生素培养基中的保存时间似乎并未影响同种异体瓣膜移除时的最终代谢活性和结构完整性。本研究结果表明，保存在汉克氏溶液中然后植入二尖瓣位置的瓣膜会出现最严重的退行性变化。以这种方式处理的瓣膜在患者体内发挥功能期间，活力百分比逐渐下降。
