Baldwin D, Crane V, Rice D
Disease Resistance Group, Trait and Technology Development, Pioneer Hi-Bred International Inc., 7300 NW 62nd Ave., Johnston, IA 50131-1004,USA.
Curr Opin Plant Biol. 1999 Apr;2(2):96-103. doi: 10.1016/S1369-5266(99)80020-X.
An initial application of plant genomics has been to monitor gene expression on a scale much larger than previously possible. Although multiplexed assays of RNA abundance have developed more quickly than those for protein and metabolite levels, some combination of these approaches will soon be providing our best views yet into plant molecular biology. Three techniques that have made contributions to the RNA transcript portion of this combination are reviewed. Currently, each can produce a profile of expression levels for a large but incomplete set of plant genes, at reproducibly high levels of accuracy and over a range of labor and financial expenses.
植物基因组学的初步应用是在比以往更大的规模上监测基因表达。尽管RNA丰度的多重检测方法比蛋白质和代谢物水平的检测方法发展得更快，但这些方法的某种组合很快将为我们提供对植物分子生物学前所未有的最佳见解。本文综述了对这种组合中RNA转录部分有贡献的三种技术。目前，每种技术都能以可重复的高精度，在不同的人力和资金成本范围内，生成大量但不完整的植物基因表达水平图谱。
