DNA芯片：曾经充满前景的新奇事物已成为强大的工具。

DNA chips: promising toys have become powerful tools.

作者信息

Gerhold D, Rushmore T, Caskey C T

机构信息

Merck & Co., Human Genetics Dept, West Point, PA 19486, USA.

出版信息

Trends Biochem Sci. 1999 May;24(5):168-73. doi: 10.1016/s0968-0004(99)01382-1.

DOI:10.1016/s0968-0004(99)01382-1

PMID:10322428

Abstract

DNA chips are glass surfaces that represent thousands of DNA fragments arrayed at discrete sites. Hybridization of RNA or DNA-derived samples to DNA chips allows us to monitor expression of mRNAs or the occurrence of polymorphisms in genomic DNA. The technology holds great promise for identifying gene polymorphisms that predispose man to disease, gene regulation events involved in disease progression, and more-effective disease treatments.

摘要

DNA芯片是玻璃表面，上面排列着成千上万个位于离散位点的DNA片段。将RNA或DNA衍生样本与DNA芯片杂交，可使我们监测mRNA的表达或基因组DNA中多态性的出现。这项技术在识别使人易患疾病的基因多态性、疾病进展过程中涉及的基因调控事件以及更有效的疾病治疗方法方面具有巨大潜力。