[用于艾滋病病毒诊断的基因芯片研发初步研究]
[Preliminary study of development of gene chips for HIV diagnosis].
作者信息
Li Ling, Ma Wen-Li, Mao Xiang-Ming, Zhu Ji, Zheng Wen-Ling
机构信息
Institute of Molecular Biology and Key Laboratory of Biochip of PLA, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2002 Aug;22(8):724-7.
OBJECTIVE
To study the technology for establishing DNA chips for the diagnosis of HIV.
METHODS
HIV 1U26942 DNA fragments were isolated by restriction display-PCR (RD-PCR) and printed onto aminosilane-coated glass slides by Pixsys 5500 arrayer as probes to prepare the gene chips. HIV samples, after labeled with Cy3, were hybridized with the microarray followed by scanning for analysis of hybridization kinetics of the RD fragments.
RESULTS
The experimental condition for preparing the gene chips was investigated and 12 RD fragments were screened as probes for further study.
CONCLUSION
The technique established in this study for preparing DNA chips is specific and applicable.
目的
研究建立用于诊断人类免疫缺陷病毒（HIV）的DNA芯片技术。
方法
通过限制性显示聚合酶链反应（RD-PCR）分离HIV 1U26942 DNA片段，并用Pixsys 5500点样仪将其点印在氨基硅烷包被的载玻片上作为探针，制备基因芯片。HIV样本用Cy3标记后，与微阵列杂交，然后进行扫描以分析RD片段的杂交动力学。
结果
研究了制备基因芯片的实验条件，筛选出12个RD片段作为探针进行进一步研究。
结论
本研究建立的制备DNA芯片的技术具有特异性和实用性。
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