Jiang G, Gu Y
Department of Hand Surgery, Huashan Hospital, Shanghai Medical University, China.
Chin Med J (Engl). 1998 Jan;111(1):63-6.
To observe the survival of embryonic motoneurons after they were transplanted into the denervated skeletal muscles and to find a new method to retard the atrophy of denervated muscles.
Dissociated embryonic motoneurons prelabled with 5-bromo-2'-deoxyuridine (Brdur) on the embryonic days 12 were injected into the denervated gastrocnemius muscles of adult rats. Then gastrocnemius muscles were processed with Nissl staining, acetylcholinesterase staining and Brdur immunocytochemical staining to show the implanted motoneurons at 9 and 22 weeks post-transplantation. Myofibrillar ATPase staining was used to show the morphology of muscle fibers. The rats in experimental group were implanted with embryonic motoneurons in the predenervation muscles, while the rats in control group were injected with just culture medium without motoneurons.
Embryonic motoneurons survived, developed and extended long axons to form neuromuscular junctions with the denervated muscles. The differentiation of muscle fibers and fiber type grouping occurred among bigger fibers in experimental group. The transverse area was smaller and there was no apparent fiber type grouping in control group.
Embryonic motoneurons can survive, develop and reinnervate denervated muscles after being transplanted into denervated muscles. It is worth further investigating on ameliorating the atrophy of denervated muscle.
观察胚胎运动神经元移植到失神经支配的骨骼肌后的存活情况，并寻找延缓失神经支配肌肉萎缩的新方法。
将在胚胎第12天用5-溴-2'-脱氧尿苷（Brdur）预标记的解离胚胎运动神经元注射到成年大鼠失神经支配的腓肠肌中。然后在移植后9周和22周对腓肠肌进行尼氏染色、乙酰胆碱酯酶染色和Brdur免疫细胞化学染色，以显示植入的运动神经元。用肌原纤维ATP酶染色显示肌纤维的形态。实验组大鼠在去神经支配前的肌肉中植入胚胎运动神经元，而对照组大鼠仅注射不含运动神经元的培养基。
胚胎运动神经元存活、发育并长出长轴突，与失神经支配的肌肉形成神经肌肉接头。实验组较大的肌纤维之间出现了肌纤维分化和纤维类型分组。对照组横截面积较小，无明显的纤维类型分组。
胚胎运动神经元移植到失神经支配的肌肉后能够存活、发育并重新支配失神经支配的肌肉。在改善失神经支配肌肉萎缩方面值得进一步研究。
