[乙型、丙型和庚型肝炎病毒多重感染的研究]

[Studies on multiple infection with hepatitis B, C and G viruses].

作者信息

Du S, Tao Q, Chang J

机构信息

Institute of Hepatology, People's Hospital, Beijing Medical University.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 1998 Jan;32(1):13-5.

PMID:10322744

Abstract

OBJECTIVE

To study if there exists superinfection and multiple infection in the patients with hepatitis G virus (HCV) infection.

METHODS

Reverse transcription polymerase chain reaction (RT-PCR) technique in non-structural gene 3 (NS3) region of HGV was used to detect HGV RNA in serially diluted quality control sera and HGV-C reference panel of samples provided by Abbott Co. Ltd, in 90 cases with positive hepatitis C virus (HCV) RNA, and 12 blood donors with super-infection of hepatitis B virus (HBV) and hepatitis C virus (HCV).

RESULTS

Serially diluted quality control sera showed positive for HGV RNA at dilutions of 10(-1) to 10(-5), but negative at dilutions of 10(-6) to 10(-8). HGV RNA was positive in two samples of HGV-C serum provided by Abbott Co. HGV RNA was positive in eight of the 90 cases with positive HCV RNA and in four of the 12 cases with super-infection with HCV and HBV.

CONCLUSION

There are not only super-infection of HBV and HCV, but also multiple infection.

摘要

目的

研究庚型肝炎病毒（HCV）感染患者中是否存在重叠感染和多重感染。

方法

采用逆转录聚合酶链反应（RT-PCR）技术，检测雅培公司提供的系列稀释的质量控制血清和HGV-C参考样本组、90例丙型肝炎病毒（HCV）RNA阳性患者以及12例乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）重叠感染的献血者血清中的HGV非结构基因3（NS3）区RNA。

结果

系列稀释的质量控制血清在10（-1）至10（-5）稀释度时HGV RNA呈阳性，在10（-6）至10（-8）稀释度时呈阴性。雅培公司提供的两份HGV-C血清样本中HGV RNA呈阳性。90例HCV RNA阳性患者中有8例HGV RNA呈阳性，12例HCV和HBV重叠感染患者中有4例HGV RNA呈阳性。