• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Effect of U-73122 on calcium channel activity in porcine myometrial cells and pancreatic beta-cell line RINm5F.

作者信息

Li S B, Chen T H, Zhu-Ge R H, Hsu W H

机构信息

Department of Veterinary Physiology and Pharmacology, Iowa State University, Ames 50011, USA.

出版信息

Zhongguo Yao Li Xue Bao. 1997 Sep;18(5):385-90.

PMID:10322922
Abstract

AIM

To study the effect of U-73122, putative phospholipase C (PLC) inhibitor, on cytosolic Ca2+ concentration ([Ca2+]i) and voltage-dependent calcium channels (VDCC).

METHODS

To record whole cell calcium current (ICa) by perforated patch-clamp and to measure cytosolic free calcium concentration ([Ca2+]i) with Fura-2.

RESULTS

U-73122 inhibited KCl-induced and Bay-K-8644-induced increase in [Ca2+]i dose-dependently. U-73122 decreased markedly depolarization-induced ICa both in RINm5F and porcine myometrial cells in a concentration-dependent manner. The effect of U-73122 was more potent in porcine myometrial cells than that in RINm5F cells. U-73343 (an analog of U-73122), that does not inhibit PLC, also reduced the ICa by 50%.

CONCLUSION

U-73122 inhibited VDCC and [Ca2+]i in both RINm5F and porcine myometrial cells.

摘要

相似文献

1
Effect of U-73122 on calcium channel activity in porcine myometrial cells and pancreatic beta-cell line RINm5F.
Zhongguo Yao Li Xue Bao. 1997 Sep;18(5):385-90.
2
Oxytocin-stimulated insulin release in a clonal beta-cell line RINm5F: involvement of phospholipase C-dependent and -independent pathways.催产素刺激克隆β细胞系RINm5F中的胰岛素释放：磷脂酶C依赖性和非依赖性途径的参与
Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):390-4.
3
Linoleic acid induces Ca2+-induced inactivation of voltage-dependent Ca2+ currents in rat pancreatic beta-cells.亚油酸诱导大鼠胰腺β细胞中电压依赖性钙电流的钙依赖性失活。
J Endocrinol. 2008 Feb;196(2):377-84. doi: 10.1677/JOE-07-0426.
4
Oxytocin induced a biphasic increase in the intracellular Ca2+ concentration of porcine myometrial cells: participation of a pertussis toxin-insensitive G-protein, inositol 1,4,5-trisphosphate-sensitive Ca2+ pool, and Ca2+ channels.催产素使猪子宫肌层细胞内钙离子浓度呈双相增加：一种对百日咳毒素不敏感的G蛋白、肌醇1,4,5-三磷酸敏感的钙离子池及钙离子通道参与其中。
Mol Reprod Dev. 1995 May;41(1):20-8. doi: 10.1002/mrd.1080410105.
5
Guanine nucleotide-sensitive inhibition of L-type Ca2+ current by lysosphingolipids in RINm5F insulinoma cells.
Mol Pharmacol. 1998 May;53(5):862-9.
6
Different mechanisms are involved in intracellular calcium increase by insulin-like growth factors 1 and 2 in articular chondrocytes: voltage-gated calcium channels, and/or phospholipase C coupled to a pertussis-sensitive G-protein.
J Cell Biochem. 1997 Mar 1;64(3):414-22.
7
In vitro effects of the cyclooxygenase inhibitor indomethacin and of the phospholipase-C inhibitor U-73122 on carbachol-induced contractions of porcine detrusor muscle.环氧化酶抑制剂吲哚美辛和磷脂酶-C抑制剂U-73122对卡巴胆碱诱导的猪逼尿肌收缩的体外作用。
Fundam Clin Pharmacol. 2008 Dec;22(6):667-72. doi: 10.1111/j.1472-8206.2008.00625.x.
8
Galanin increases cytoplasmic calcium in insulin-producing RINm5F cells by activating phospholipase C.甘丙肽通过激活磷脂酶C增加胰岛素分泌细胞RINm5F中的细胞质钙。
Biochem Biophys Res Commun. 1996 Apr 5;221(1):89-94. doi: 10.1006/bbrc.1996.0550.
9
Alpha2-adrenergic receptor-mediated Ca2+ influx and release in porcine myometrial cells.α2肾上腺素能受体介导的猪子宫肌层细胞Ca2+内流和释放
Biol Reprod. 1997 May;56(5):1343-50. doi: 10.1095/biolreprod56.5.1343.
10
Effect of thimerosal on Ca(2+) movement and viability in human oral cancer cells.硫柳汞对人口腔癌细胞中Ca(2+) 移动及细胞活力的影响。
Hum Exp Toxicol. 2009 May;28(5):301-8. doi: 10.1177/0960327109106548. Epub 2009 Aug 6.