Yin X, Zhu Y H, Xu S F
Department of Neurobiology, Shanghai Medical University, China.
Zhongguo Yao Li Xue Bao. 1997 Sep;18(5):391-4.
To compare the expressions of prepropiomelanocortin (POMC) mRNA and preprodynorphin (PPD) mRNA between 16-wk-old spontaneously hypertensive rats (SHR) and age-matched normotensive Wistar-Kyoto rats (WKY).
The expression of POMC mRNA and PPD mRNA were detected with nonradioactive in situ hybridization by digoxigenin-labeled RNA probe.
POMC mRNA mainly was expressed in arcuate nucleus, compared with WKY, SHR had higher level of POMC mRNA (542). PPD mRNA was found in hippocampus, hypothalamus, central gray, nucleus of the solitary tract (NTS), and thoracic spinal cord (T4-T6). Compared with WKY, PPD mRNA level of SHR decreased in dentate gyrus (2342), NTS (381), and medial preoptic area (467); no difference was observed in arcuate nucleus (263), thoracic spinal cord (750-1800) and CA1, CA2, CA3 of hippocampus (1674, 2014, 2626).
Increase of POMC mRNA in arcuate nucleus and decrease of PPD mRNA in dentate gyrus of SHR may be associated with the genesis of spontaneous hypertension.
比较16周龄自发性高血压大鼠（SHR）与年龄匹配的正常血压Wistar-Kyoto大鼠（WKY）中阿黑皮素原（POMC）mRNA和前强啡肽原（PPD）mRNA的表达。
采用地高辛标记的RNA探针，通过非放射性原位杂交检测POMC mRNA和PPD mRNA的表达。
POMC mRNA主要在弓状核表达，与WKY相比，SHR的POMC mRNA水平更高（542）。PPD mRNA在海马、下丘脑、中脑导水管周围灰质、孤束核（NTS）和胸段脊髓（T4-T6）中被发现。与WKY相比，SHR的PPD mRNA水平在齿状回（2342）、NTS（381）和视前内侧区（467）降低；在弓状核（263）、胸段脊髓（750-1800）以及海马的CA1、CA2、CA3区（1674、2014、2626）未观察到差异。
SHR弓状核中POMC mRNA增加以及齿状回中PPD mRNA减少可能与自发性高血压的发生有关。
