降钙素基因相关肽诱导的预处理对溶血磷脂酰胆碱诱导的内皮依赖性血管舒张减弱的影响。

Effect of calcitonin gene-related peptide-induced preconditioning on attenuated endothelium-dependent vasorelaxation induced by lysophosphatidylcholine.

作者信息

Tang Y H, Lu R, Li Y J, Peng C F, Deng H W

机构信息

Department of Pharmacology, Hunan Medical University, Changsha, China.

出版信息

Zhongguo Yao Li Xue Bao. 1997 Sep;18(5):405-7.

PMID:10322927

Abstract

AIM

To study the effects of calcitonin gene-related peptide (CGRP)-induced preconditioning on the inhibition of endothelium-dependent vasorelaxation to acetylcholine (ACh) by lysophosphatidylcholine (Lys) in the isolated rabbit and rat thoracic aortas.

METHODS

Endothelium-dependent relaxation to ACh was studied in the aortic rings precontracted with phenylephrine 0.1 mumol.L-1 in the absence or presence of Lys.

RESULTS

On the rabbit aortic rings, Lys 5 mg.L-1 impaired vasodilator responses to ACh. Pretreatment with CGRP 0.1 mumol.L-1 for 5 min attenuated the inhibition of vasodilator responses to ACh by Lys. The effect of CGRP was blocked by 1-(5-isoquinolinysulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein kinase C (PKC) (% relaxations to ACh 1 mumol.L-1 were 88 +/- 4, 28 +/- 10, 65 +/- 13, and 25 +/- 10 for control, Lys, Lys + CGRP, and Lys + CGRP + H-7, respectively). The same effects of CGRP were shown in the rat aortic rings, and the effect of CGRP was also abolished by H-7 (% relaxations to ACh 1 mumol.L-1 was 84 +/- 10, 55 +/- 11, 76 +/- 11, and 50 +/- 14 for control, Lys, Lys + CGRP, and Lys + CGRP + H-7, respectively).

CONCLUSION

CGRP-induced preconditioning protected the endothelium against injury elicited by Lys, the effect of CGRP is related to the activation of PKC.

摘要

目的

研究降钙素基因相关肽（CGRP）诱导的预处理对溶血磷脂酰胆碱（Lys）抑制离体兔和大鼠胸主动脉内皮依赖性血管舒张反应（对乙酰胆碱（ACh）的反应）的影响。

方法

在存在或不存在Lys的情况下，研究用0.1 μmol·L-1去氧肾上腺素预收缩的主动脉环对ACh的内皮依赖性舒张反应。

结果

在兔主动脉环上，5 mg·L-1的Lys损害了对ACh的血管舒张反应。用0.1 μmol·L-1 CGRP预处理5分钟可减弱Lys对ACh血管舒张反应的抑制作用。CGRP的作用被蛋白激酶C（PKC）抑制剂1-（5-异喹啉磺酰基）-2-甲基哌嗪（H-7）阻断（对1 μmol·L-1 ACh的舒张率分别为：对照组88±4、Lys组28±10、Lys + CGRP组65±13、Lys + CGRP + H-7组25±10）。在大鼠主动脉环上也显示出CGRP的相同作用，且H-7也消除了CGRP的作用（对1 μmol·L-1 ACh的舒张率分别为：对照组84±10、Lys组55±11、Lys + CGRP组76±11、Lys + CGRP + H-7组50±14）。