Tang Y H, Lu R, Li Y J, Peng C F, Deng H W
Department of Pharmacology, Hunan Medical University, Changsha, China.
Zhongguo Yao Li Xue Bao. 1997 Sep;18(5):405-7.
To study the effects of calcitonin gene-related peptide (CGRP)-induced preconditioning on the inhibition of endothelium-dependent vasorelaxation to acetylcholine (ACh) by lysophosphatidylcholine (Lys) in the isolated rabbit and rat thoracic aortas.
Endothelium-dependent relaxation to ACh was studied in the aortic rings precontracted with phenylephrine 0.1 mumol.L-1 in the absence or presence of Lys.
On the rabbit aortic rings, Lys 5 mg.L-1 impaired vasodilator responses to ACh. Pretreatment with CGRP 0.1 mumol.L-1 for 5 min attenuated the inhibition of vasodilator responses to ACh by Lys. The effect of CGRP was blocked by 1-(5-isoquinolinysulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein kinase C (PKC) (% relaxations to ACh 1 mumol.L-1 were 88 +/- 4, 28 +/- 10, 65 +/- 13, and 25 +/- 10 for control, Lys, Lys + CGRP, and Lys + CGRP + H-7, respectively). The same effects of CGRP were shown in the rat aortic rings, and the effect of CGRP was also abolished by H-7 (% relaxations to ACh 1 mumol.L-1 was 84 +/- 10, 55 +/- 11, 76 +/- 11, and 50 +/- 14 for control, Lys, Lys + CGRP, and Lys + CGRP + H-7, respectively).
CGRP-induced preconditioning protected the endothelium against injury elicited by Lys, the effect of CGRP is related to the activation of PKC.
研究降钙素基因相关肽（CGRP）诱导的预处理对溶血磷脂酰胆碱（Lys）抑制离体兔和大鼠胸主动脉内皮依赖性血管舒张反应（对乙酰胆碱（ACh）的反应）的影响。
在存在或不存在Lys的情况下，研究用0.1 μmol·L-1去氧肾上腺素预收缩的主动脉环对ACh的内皮依赖性舒张反应。
在兔主动脉环上，5 mg·L-1的Lys损害了对ACh的血管舒张反应。用0.1 μmol·L-1 CGRP预处理5分钟可减弱Lys对ACh血管舒张反应的抑制作用。CGRP的作用被蛋白激酶C（PKC）抑制剂1-（5-异喹啉磺酰基）-2-甲基哌嗪（H-7）阻断（对1 μmol·L-1 ACh的舒张率分别为：对照组88±4、Lys组28±10、Lys + CGRP组65±13、Lys + CGRP + H-7组25±10）。在大鼠主动脉环上也显示出CGRP的相同作用，且H-7也消除了CGRP的作用（对1 μmol·L-1 ACh的舒张率分别为：对照组84±10、Lys组55±11、Lys + CGRP组76±11、Lys + CGRP + H-7组50±14）。
CGRP诱导的预处理可保护内皮免受Lys引起的损伤，CGRP的作用与PKC的激活有关。
