重组人内皮衍生白细胞介素-8对大鼠失血性休克的影响。
Effects of recombinant human endothelial-derived interleukin-8 on hemorrhagic shock in rats.
作者信息
Wang L Z, Su J Y, Lu C Y, Zhou B H, Ma D L
机构信息
Institute of Cardiovascular Basic Research, Beijing Medical University, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 Sep;18(5):434-6.
AIM
To study the effects of recombinant human endothelial-derived interleukin-8 (IL-8) on hemorrhagic shock.
METHODS
A profound hemorrhagic shock in rats was produced by exsanguination from femoral artery with mean arterial blood pressure (MABP) maintained at 5.32 kPa for 90 min. After transfusion, IL-8 250 micrograms.kg-1 was i.v. injected. Plasma endothelin-1 (ET-1) and 6 ketoprostaglandin F1 alpha (6-KPGF1 alpha) contents were determined with radioimmunoassay.
RESULTS
After i.v. IL-8, the MABP in IL-8 group was elevated obviously (P < 0.01), the rat survival 2 h after infusion was increased (P < 0.05). During profound shock the plasma ET-1 levels were higher (21 +/- 4 vs 8.2 +/- 1.8 ng.L-1, P < 0.01) and the plasma 6-KPGF1 alpha contents lower than those in normal rats (107 +/- 12 vs 157 +/- 11 ng.L-1, P < 0.01). IL-8 remarkably reduced the plasma ET-1 levels (10 +/- 4 ng.L-1, P < 0.01) and enhanced plasma 6-KPGF1 alpha contents (368 +/- 16 ng.L-1, P < 0.01).
CONCLUSION
IL-8 has beneficial antishock effects.
目的
研究重组人内皮源性白细胞介素-8（IL-8）对失血性休克的影响。
方法
通过股动脉放血使大鼠产生严重失血性休克，将平均动脉血压（MABP）维持在5.32 kPa 90分钟。输血后，静脉注射250微克·千克-1的IL-8。采用放射免疫分析法测定血浆内皮素-1（ET-1）和6-酮前列腺素F1α（6-KPGF1α）含量。
结果
静脉注射IL-8后，IL-8组的MABP明显升高（P<0.01），输液后2小时大鼠存活率增加（P<0.05）。在严重休克期间，血浆ET-1水平较高（21±4对8.2±1.8纳克·升-1，P<0.01），血浆6-KPGF1α含量低于正常大鼠（107±12对157±11纳克·升-1，P<0.01）。IL-8显著降低血浆ET-1水平（10±4纳克·升-1，P<0.01），并提高血浆6-KPGF1α含量（368±16纳克·升-1，P<0.01）。
结论
IL-8具有有益的抗休克作用。
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