Jiang B, Guo J Y
Department of Forensic Medicine, Sun-Yat Sen University of Medicine Science.
Fa Yi Xue Za Zhi. 1997;13(2):68-70, 128.
Sex determination is carried out by simultaneously amplifying a X-specific and Y-specific DNA fragments which are 106 bp and 112 bp long respectively in one PCR reaction using one pair of primers which are specific to the first intron of the X-Y homologous gene Amelogenin PCR products are separated on a PAGE electrophoresis followed by visulization using silver stain. All the blood stain, muscle, saliva and hair samples gave correct sex determination. Less than or as little as 50 pg template DNA and the 16-year-old blood stain all can achieve satisfactory amplification. The results showed that the method discribed in this paper is a rapid, sensitive, reliable and easily manipulated sex test highly adapted to forensic specimen especially those petrified and degraded.
性别鉴定是通过在一个聚合酶链反应（PCR）中，使用一对针对X-Y同源基因牙釉蛋白第一内含子的引物，同时扩增分别为106 bp和112 bp长的X特异性和Y特异性DNA片段来进行的。PCR产物在聚丙烯酰胺凝胶电泳（PAGE）上分离，随后用银染法进行可视化。所有血迹、肌肉、唾液和毛发样本都给出了正确的性别鉴定结果。少于或低至50 pg的模板DNA以及16年的陈旧血迹都能实现令人满意的扩增。结果表明，本文所述方法是一种快速、灵敏、可靠且易于操作的性别检测方法，非常适用于法医样本，尤其是那些石化和降解的样本。
