Fukumasu Y, Fukumasu H, Yamamasu S, Tachibana D, Imanaka M, Ogita S
Department of Obstetrics and Gynecology, Osaka City University Medical School, Osaka, Japan.
Biol Neonate. 1999;75(6):367-76. doi: 10.1159/000014117.
The small neutrophil reserve and exaggerated release of stored neutrophils are factors which predispose neonates to neutrophil reserve exhaustion during bacterial sepsis. Our objective is to try to improve in utero the myelopoietic function of the fetus before delivery. In the first series, recombinant human (rh) granulocyte colony-stimulating factor (G-CSF) (rhG-CSF; 100 microg/kg) was injected subcutaneously into rat fetuses at the indicated times to assess drug absorption and fetal response. In the second series, rhG-CSF (100 microg/kg) or saline (control) was injected into the fetuses once every other day to investigate the effect of repeated injections of rhG-CSF on enhancing fetal myelopoiesis preceding birth. Delivery was performed by cesarean section on embryonic day 21. The plasma concentration of G-CSF was determined by ELISA. The effect of rhG-CSF injection on granulopoiesis was assessed by measurement of the neutrophil count in the fetal peripheral blood and by histological examination of the fetal bone marrow, spleen, and liver. Fetally administered rhG-CSF enhanced fetal myelopoiesis preceding birth.
少量的中性粒细胞储备以及储存的中性粒细胞的过度释放是导致新生儿在细菌性败血症期间中性粒细胞储备耗竭的因素。我们的目标是在分娩前尝试改善胎儿在子宫内的骨髓造血功能。在第一组实验中，在指定时间将重组人（rh）粒细胞集落刺激因子（G-CSF）（rhG-CSF；100μg/kg）皮下注射到大鼠胎儿体内，以评估药物吸收和胎儿反应。在第二组实验中，每隔一天给胎儿注射一次rhG-CSF（100μg/kg）或生理盐水（对照），以研究重复注射rhG-CSF对增强出生前胎儿骨髓造血的影响。在胚胎第21天通过剖宫产进行分娩。通过酶联免疫吸附测定法测定G-CSF的血浆浓度。通过测量胎儿外周血中的中性粒细胞计数以及对胎儿骨髓、脾脏和肝脏进行组织学检查来评估rhG-CSF注射对粒细胞生成的影响。在出生前，经胎儿给药的rhG-CSF可增强胎儿的骨髓造血功能。
