Park W S, Chang Y S, Ko S Y, Kang M J, Han J M, Lee M
Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, Korea.
Biol Neonate. 1999;75(6):377-87. doi: 10.1159/000014118.
This study was done to evaluate the efficacy of anti-tumor necrosis factor alpha (anti-TNF-alpha) antibody as an adjunctive therapy in neonatal bacterial meningitis. Newborn piglets were divided into three groups: 8 in the control group, 13 in the meningitis group (MG), and 10 in the meningitis with anti-TNF-alpha antibody group (AG). Meningitis was induced by intracisternal injection of 10(8) colony-forming units of Escherichia coli in 100 microl of saline. In the AG, 200 microl of anti-TNF-alpha antibody was also given intracisternally. In the AG, the elevated cerebrospinal fluid TNF-alpha level observed in the MG was completely abolished, and increased intracranial pressure, hypoglycorrhachia, and CSF pleocytosis observed in the MG were downmodulated. But blood, brain, and CSF lactate levels remained elevated in both MG and AG. Increased brain cell membrane lipid peroxidation products and decreased Na+,K+-ATPase activity observed in the MG were not attenuated in the AG. These results indicate that anti-TNF-alpha antibody was not particularly effective as an adjunctive therapy in attenuating acute inflammatory responses and ameliorating brain damage in neonatal bacterial meningitis.
本研究旨在评估抗肿瘤坏死因子α（抗TNF-α）抗体作为新生儿细菌性脑膜炎辅助治疗的疗效。新生仔猪被分为三组：对照组8只，脑膜炎组（MG）13只，抗TNF-α抗体脑膜炎组（AG）10只。通过向脑池内注射100微升含10(8)个大肠杆菌菌落形成单位的生理盐水诱导脑膜炎。在AG组中，也向脑池内注射200微升抗TNF-α抗体。在AG组中，MG组中观察到的脑脊液TNF-α水平升高被完全消除，MG组中观察到的颅内压升高、脑脊液糖降低和脑脊液细胞增多症得到下调。但MG组和AG组的血液、脑和脑脊液乳酸水平均保持升高。MG组中观察到的脑细胞膜脂质过氧化产物增加和Na +,K + -ATP酶活性降低在AG组中未得到缓解。这些结果表明，抗TNF-α抗体作为辅助治疗在减轻新生儿细菌性脑膜炎的急性炎症反应和改善脑损伤方面并非特别有效。
