Wilkinson N Z, Kingsley G H, Jones H W, Sieper J, Braun J, Ward M E
Molecular Microbiology, University of Southampton, UK.
Rheumatology (Oxford). 1999 Mar;38(3):260-6. doi: 10.1093/rheumatology/38.3.260.
Bacteria have been implicated in the pathogenesis of many types of inflammatory arthritides. The aim of this study was to identify any bacterial DNA in synovial fluid (SF) from patients with a range of inflammatory arthritides.
A highly sensitive, broad-range, nested polymerase chain reaction (PCR) protocol targeting the bacterial 16S rRNA gene was designed and applied to SF from 65 patients with a range of rheumatic diseases.
Bacterial DNA was detected in 26 SF samples, including eight from patients with rheumatoid arthritis and five with juvenile arthritides. PCR products were identified by sequencing and searching of bacterial genomic databases; 'best fits' included Haemophilus influenzae, Bordetella and Yersinia.
These finding suggest an association between bacterial infection and inflammatory arthritides in some patients. Further research is required to determine the role of these organisms in the pathogenesis and whether such patients might respond to prolonged antibiotic therapy.
细菌已被认为与多种类型的炎性关节炎的发病机制有关。本研究的目的是在一系列炎性关节炎患者的滑液（SF）中鉴定任何细菌DNA。
设计了一种针对细菌16S rRNA基因的高灵敏度、宽范围、巢式聚合酶链反应（PCR）方案，并将其应用于65例患有多种风湿性疾病患者的滑液中。
在26份滑液样本中检测到细菌DNA，其中包括8份来自类风湿性关节炎患者和5份来自青少年关节炎患者的样本。通过对细菌基因组数据库进行测序和搜索来鉴定PCR产物；“最佳匹配”包括流感嗜血杆菌、博德特氏菌和耶尔森氏菌。
这些发现表明在一些患者中细菌感染与炎性关节炎之间存在关联。需要进一步研究以确定这些微生物在发病机制中的作用以及此类患者是否可能对长期抗生素治疗有反应。
