Zalan E, Wilson C
Can Med Assoc J. 1976 Nov 20;115(10):1002-3.
With the use of bacteria sensitized by influenza virus strain-specific antisera, virus isolates can be identified rapidly. One drop of virus suspension is mixed with one drop of sensitized bacteria on a slide that is then agitated; reaction occurs within 10 minutes. The test is subtype-specific. The mehod is based on the fact that the cell wall of the Cowan type 1 strain of Staphylococcus aureus contains abundant quantities of an antigen, known as protein A, that reacts with the IgG molecule by binding it in such a manner that the antibody-combining sites remain free. If an antigen homologous to the antibody coated on the surface of the bacteria is added to the suspension of sensitized staphylococci, agglutination occurs.
通过使用经流感病毒株特异性抗血清致敏的细菌，可以快速鉴定病毒分离株。将一滴病毒悬液与一滴致敏细菌在载玻片上混合，然后搅拌；反应在10分钟内发生。该试验具有亚型特异性。该方法基于这样一个事实，即金黄色葡萄球菌考恩1型菌株的细胞壁含有大量一种名为蛋白A的抗原，它通过以一种使抗体结合位点保持游离的方式与IgG分子反应。如果将与包被在细菌表面的抗体同源的抗原添加到致敏葡萄球菌悬液中，就会发生凝集。
