Ishida T, Hirata K, Sakoda T, Kawashima S, Akita H, Yokoyama M
First Department of Internal Medicine, Kobe University School of Medicine, Japan.
Cardiovasc Res. 1999 Jan;41(1):267-74. doi: 10.1016/s0008-6363(98)00162-x.
Although pharmacological studies have indicated that serotonin (5-HT)-evoked contraction of the human coronary artery is mediated by 5-HT1-like and 5-HT2 receptors, the gene expression of 5-HT receptors is still unclear. We examined mRNA expression of 5-HT1 and 5-HT2 receptor subtypes in human coronary arteries.
Total RNA was extracted from human coronary arteries of 14 patients at autopsy by the guanidine method. Reverse transcription-polymerase chain reaction (RT-PCR) and ribonuclease protection assays were performed to identify 5-HT1 and 5-HT2 receptor mRNA expression in human coronary artery.
By RT-PCR, 5-HT1b, 5-HT2A and 5-HT2B mRNAs were detected in all of the 14 patients. 5-HT1A, 5-HT1D, and 5-HT1E mRNAs were detected in only some patients. However, neither 5-HT1F mRNA nor 5-HT2C mRNA was detected in any patient. By ribonuclease protection assay, 5-HT1B and 5-HT2A signals were detected in all patients examined, but neither 5-HT1A, 5-HT1D nor 5-HT2B signal was detected in any patient.
Of 5-HT1/2 receptor subtypes, 5-HT1B and 5-HT2A receptor mRNAs were predominantly expressed in human coronary arteries. Our finding provides molecular evidence that the 5-HT1B receptor may be the 5-HT1-like receptor which mediates constriction of human coronary arteries.
尽管药理学研究表明，血清素（5-羟色胺，5-HT）诱发的人类冠状动脉收缩是由5-HT1样受体和5-HT2受体介导的，但5-HT受体的基因表达仍不清楚。我们检测了人类冠状动脉中5-HT1和5-HT2受体亚型的mRNA表达。
采用胍法从14例尸检患者的冠状动脉中提取总RNA。进行逆转录-聚合酶链反应（RT-PCR）和核糖核酸酶保护试验，以鉴定人类冠状动脉中5-HT1和5-HT2受体mRNA的表达。
通过RT-PCR，在所有14例患者中均检测到5-HT1b、5-HT2A和5-HT2B mRNA。仅在部分患者中检测到5-HT1A、5-HT1D和5-HT1E mRNA。然而，在任何患者中均未检测到5-HT1F mRNA和5-HT2C mRNA。通过核糖核酸酶保护试验，在所有检测患者中均检测到5-HT1B和5-HT2A信号，但在任何患者中均未检测到5-HT1A、5-HT1D和5-HT2B信号。
在5-HT1/2受体亚型中，5-HT1B和5-HT2A受体mRNA在人类冠状动脉中主要表达。我们的发现提供了分子证据，表明5-HT1B受体可能是介导人类冠状动脉收缩的5-HT1样受体。
