Leblebicioglu H, Nas Y, Eroglu C, Sunbul M, Esen S, Gunaydin M
Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayis University, Medical School, Samsun, Turkey.
J Chemother. 1999 Apr;11(2):103-6. doi: 10.1179/joc.1999.11.2.103.
The presence of extended spectrum beta-lactamases (ESbetaLs) in 129 Escherichia coli and 128 Klebsiella pneumoniae strains was determined with the E test. beta-lactamases were detected in 66 (51.2%) of E.coli and 107 (83.6%) of K. pneumoniae by the nitrocefin disk method. Putative ESbetaL production was observed in 15.5% of E. coli and 55.5% of K. pneumoniae with the E test. Among the putative ESbetaL-producer strains 11 were found to be susceptible to ceftizoxime, 6 to ceftazidime, 2 to ceftriaxone, cefotaxime and aztreonam, and one to cefoperazone according to the disk diffusion test. All putative ESbetaL-producers were resistant to cefodizime, thus it may be a good indicator of the presence of mainly TEM-type ESbetaL. The E test was also found to be practicable for the detection of ESbetaLs in clinical laboratory. Further studies are needed to clarify whether ESbetaL can be identified reliably in species that, in addition to plasmid mediated ESbetaL production, chromosomally encoded AmpC beta-lactamases and other class A ESbetaLs by disk diffusion test.
采用E试验检测了129株大肠杆菌和128株肺炎克雷伯菌中广谱β-内酰胺酶（ESbetaLs）的存在情况。通过硝基头孢菌素纸片法在66株（51.2%）大肠杆菌和107株（83.6%）肺炎克雷伯菌中检测到β-内酰胺酶。用E试验在15.5%的大肠杆菌和55.5%的肺炎克雷伯菌中观察到推定的ESbetaL产生。根据纸片扩散试验，在推定的ESbetaL产生菌中，发现11株对头孢唑肟敏感，6株对头孢他啶敏感，2株对头孢曲松、头孢噻肟和氨曲南敏感，1株对头孢哌酮敏感。所有推定的ESbetaL产生菌均对头孢地嗪耐药，因此它可能是主要存在TEM型ESbetaL的一个良好指标。还发现E试验在临床实验室检测ESbetaLs方面是可行的。需要进一步研究以阐明通过纸片扩散试验在除质粒介导的ESbetaL产生外还存在染色体编码的AmpCβ-内酰胺酶和其他A类ESbetaLs的菌种中能否可靠地鉴定ESbetaL。
