Stoeva S, Dolashka P, Hristova R, Genov N, Voelter W
Abteilung für Physikalische Biochemie, Physiologisch-chemisches Institut der Universität Tübingen, Germany.
Comp Biochem Physiol B Biochem Mol Biol. 1999 Jan;122(1):69-75. doi: 10.1016/s0305-0491(98)10144-x.
Fourteen structural subunits of hemocyanins from two different infraorders of crustacea, brachyura (Maia squinado, Carcinus maenas) and astacidea (Homarus americanus) were isolated and characterized. N-terminal amino acid sequences were determined and compared with known sequences of crustacean and cheliceratan hemocyanins. Relationships between the investigated polypeptide chains were established. The results demonstrate that the degree of identity, calculated from the amino terminal sequences, is lower than that determined from the complete sequences and can be used for reliable characterization of the whole individual subunits. Independent evolution is possible not only for members of different subphyla, but also for those from one infraorder or from the same hemocyanin.
从甲壳纲两个不同下目的血蓝蛋白中分离并鉴定出14个结构亚基，这两个下目分别是短尾派（黄道蟹、滨蟹）和螯虾派（美洲螯龙虾）。测定了N端氨基酸序列，并与甲壳类和螯肢动物血蓝蛋白的已知序列进行了比较。确定了所研究多肽链之间的关系。结果表明，根据氨基末端序列计算出的同一性程度低于根据完整序列确定的程度，并且可用于对整个单个亚基进行可靠的表征。不仅不同亚门的成员，而且来自一个下目或同一血蓝蛋白的成员都可能发生独立进化。
