Hijri M, Hosny M, van Tuinen D, Dulieu H
Laboratoire de Phytoparasitologie, INRA/CNRS, CMSE-INRA, BV 1540, Dijon Cedex, 21034, France.
Fungal Genet Biol. 1999 Mar;26(2):141-51. doi: 10.1006/fgbi.1998.1112.
Polymorphism of the internal transcribed spacers (ITS) of the ribosomal DNA in Scutellospora castanea (Glomales, Zygomycota) and its organization among spores were evaluated. Polymerase chain reaction (PCR) amplification with ITS1/ITS4 primers yielded several fragments of different lengths, even from single spores. Fragments produced from multisporal DNA were cloned and grouped into 6 ITS types by PCR-RFLP and sequence analysis. Five type-specific primers were designed. Spores were then analyzed by PCR and amplification profiles revealed that they were qualitatively different one from another due to the presence or absence of some ITS types. Intrasporal segregation of ITS variant length types was also shown, by PCR experiments, utilizing diluted fractions of nuclear suspensions from single spores. The results demonstrate the mainly multikaryotic condition of the spores of S. castanea.
对栗色盾巨孢囊霉（球囊霉目，接合菌门）核糖体DNA的内转录间隔区（ITS）多态性及其在孢子中的组织情况进行了评估。使用ITS1/ITS4引物进行聚合酶链反应（PCR）扩增，即使从单个孢子也能产生几个不同长度的片段。从多孢子DNA产生的片段进行克隆，并通过PCR-RFLP和序列分析分为6种ITS类型。设计了5种类型特异性引物。然后通过PCR分析孢子，扩增图谱显示由于某些ITS类型的存在或缺失，它们在质量上彼此不同。利用单个孢子核悬浮液的稀释部分进行的PCR实验也表明了ITS变异长度类型的孢子内分离。结果证明了栗色盾巨孢囊霉孢子主要为多核状态。
