Heinemann T, Bulwin G C, Randall J, Schnieders B, Sandhoff K, Volk H D, Milford E, Gullans S R, Utku N
Kekulé-Institut für Organische Chemie und Biochemie, Universität Bonn, Bonn, 53121, Germany.
Genomics. 1999 May 1;57(3):398-406. doi: 10.1006/geno.1999.5751.
A novel human membrane protein, TIRC7, was recently identified and demonstrated to be essential in T cell activation. Here we report on the genomic organization of the TIRC7 gene, which is composed of 15 exons and spans 7.9 kb. The seven predicted transmembrane-spanning domains of the TIRC7 protein coincide well with exon-intron boundaries. TIRC7 and OC116, a recently described putative subunit of the vacuolar proton pump that was demonstrated to be expressed in an osteoclastoma tumor as well as in a human pancreatic adenocarcinoma cell line, are demonstrated to be alternative transcripts of the same gene. OC116 consists of 20 exons with the last 14 introns and exons being identical with those of TIRC7. The chromosomal locus for both transcripts was identified on chromosome 11q13.4-q13.5. In human alloactivated T lymphocytes, mRNA expression of TIRC7, but not OC116, is demonstrated, indicating that OC116 is not involved in regular T cell proliferation.
一种新型人类膜蛋白TIRC7最近被鉴定出来，并被证明在T细胞活化中至关重要。在此，我们报告TIRC7基因的基因组结构，该基因由15个外显子组成，跨度为7.9 kb。TIRC7蛋白的七个预测跨膜结构域与外显子 - 内含子边界吻合良好。TIRC7和OC116被证明是同一基因的可变转录本，OC116是液泡质子泵最近描述的一个假定亚基，已证实在破骨细胞瘤肿瘤以及人胰腺腺癌细胞系中表达。OC116由20个外显子组成，最后14个内含子和外显子与TIRC7的相同。两种转录本的染色体定位均在11号染色体q13.4 - q13.5上被确定。在人类同种异体活化的T淋巴细胞中，证实有TIRC7的mRNA表达，但没有OC116的表达，这表明OC116不参与正常T细胞增殖。
