Soda Y, Shimizu N, Jinno A, Liu H Y, Kanbe K, Kitamura T, Hoshino H
Department of Hygiene and Virology, Gunma University School of Medicine, Gunma, Japan.
Biochem Biophys Res Commun. 1999 May 10;258(2):313-21. doi: 10.1006/bbrc.1999.0633.
CD4 and one of the G-protein-coupled receptors (GPCRs) on the cell surface function as a receptor and a coreceptor, respectively, in infection of cells with human and simian immunodeficiency viruses (HIV/SIV). To determine which GPCRs can be coreceptors for HIV (HIV-1 and HIV-2) or SIV infection, several cell lines, including human osteosarcoma HOS-T4 cells and human glioma U87/CD4 cells, have been used. However, these cells often show susceptibilities to some HIV or SIV strains before transduction of GPCRs. The results of this study showed that a CD4-transduced human glioma cell line, NP-2/CD4, a human erythroleukemia cell line, K562/CD4, and a human ovarian cancer cell line, TYK/CD4, were completely resistant to the HIV-1 and HIV-2 strains tested. After transduction of several GPCRs into NP-2/CD4, K562/CD4, or TYK/CD4 cells, NP-2/CD4 cells but not K562/CD4 or TYK/CD4 cells mostly showed expected susceptibilities to several HIV strains. Therefore, an NP-2 cell system would be useful to determine the coreceptor usage of HIV isolates, to find a new coreceptor for HIV/SIV, and to analyze the early stages of HIV/SIV infection.
在人类和猿猴免疫缺陷病毒（HIV/SIV）感染细胞的过程中，CD4和细胞表面的一种G蛋白偶联受体（GPCR）分别作为受体和共受体发挥作用。为了确定哪些GPCR可以作为HIV（HIV-1和HIV-2）或SIV感染的共受体，人们使用了多种细胞系，包括人骨肉瘤HOS-T4细胞和人胶质瘤U87/CD4细胞。然而，这些细胞在转导GPCR之前，往往对某些HIV或SIV毒株表现出易感性。本研究结果表明，转导了CD4的人胶质瘤细胞系NP-2/CD4、人红白血病细胞系K562/CD4和人卵巢癌细胞系TYK/CD4，对所测试的HIV-1和HIV-2毒株完全耐药。在将几种GPCR转导到NP-2/CD4、K562/CD4或TYK/CD4细胞中后，NP-2/CD4细胞而非K562/CD4或TYK/CD代表了几种HIV毒株的预期易感性。因此，NP-2细胞系统将有助于确定HIV分离株的共受体使用情况，寻找HIV/SIV的新共受体，并分析HIV/SIV感染的早期阶段。
