一种用于大规模DNA测序的自动化样本制备系统。
An automated sample preparation system for large-scale DNA sequencing.
作者信息
Marziali A, Willis T D, Federspiel N A, Davis R W
机构信息
Stanford DNA Sequencing and Technology Center, Palo Alto, California 94304, USA.
出版信息
Genome Res. 1999 May;9(5):457-62.
Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.
DNA测序技术的最新进展，无论是高泳道密度凝胶形式还是自动化毛细管系统形式，都将导致对以低成本和高通量运行的样品制备系统的需求增加。作为全自动测序系统开发的一部分，我们已经开发了一个自动化子系统，该子系统能够每天从M13噬菌斑文库中制备出10,000个可用于测序的单链DNA模板，每个样品的成本为0.29美元。这个前端自1997年6月以来一直在高通量运行，已经生产了超过400,000个高质量的DNA模板。
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