ACAPELLA-1K，一种用于基因组分析的基于毛细管的亚微升自动流体处理系统。
ACAPELLA-1K, a capillary-based submicroliter automated fluid handling system for genome analysis.
作者信息
Meldrum D R, Evensen H T, Pence W H, Moody S E, Cunningham D L, Wiktor P J
机构信息
Department of Electrical Engineering, Genomation Laboratory, University of Washington, Seattle, Washington 98195-2500 USA.
出版信息
Genome Res. 2000 Jan;10(1):95-104.
The Genomation Laboratory in the Electrical Engineering Department at the University of Washington has been developing an automated, high-throughput, submicroliter-scale fluid-handling system for use in molecular biology, especially as part of the Human Genome Project and other high-throughput DNA sequencing endeavors. Small glass capillaries enable the preparation, handling, and monitoring of 1-microliter reaction volumes. The Genomation Laboratory, with corporate partners Orca Photonic Systems, Inc. and Engineering Arts, has developed modules for aspiration, dispensing, mixing, transport, and rapid thermal processing of biological samples contained in glass capillaries. The ACAPELLA-1K is the first integration of these modules, designed to process 1000 samples in an eight-hour day. It has served as a test bed for the technologies as well as for performing biological experiments in conjunction with the University of Washington Genome Center. This system and related results are presented in this paper. A video of the system in operation is provided at. The Genomation Laboratory is presently developing the next-stage ACAPELLA-5K system based on the results of the ACAPELLA-1K system.
华盛顿大学电气工程系的基因组实验室一直在开发一种自动化、高通量、亚微升规模的流体处理系统，用于分子生物学领域，特别是作为人类基因组计划和其他高通量DNA测序工作的一部分。小型玻璃毛细管能够对1微升反应体积进行制备、处理和监测。基因组实验室与企业合作伙伴奥卡光子系统公司和工程艺术公司共同开发了用于抽吸、分配、混合、运输以及对玻璃毛细管中所含生物样品进行快速热处理的模块。ACAPELLA - 1K是这些模块的首次集成，设计为每天八小时可处理1000个样品。它已成为这些技术的试验台，同时也与华盛顿大学基因组中心一起用于进行生物学实验。本文介绍了该系统及相关结果。文中提供了该系统运行的视频。基因组实验室目前正在基于ACAPELLA - 1K系统的结果开发下一阶段的ACAPELLA - 5K系统。
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