Gandley R, Anderson L, Silbergeld E K
Program in Toxicology, University of Maryland, 660 West Redwood Street, Baltimore, Maryland 21201, USA.
Environ Res. 1999 May;80(4):355-63. doi: 10.1006/enrs.1998.3874.
The present study was designed to determine the effect of relatively low levels of lead acetate (25 and 250 ppm) exposure on fertility and offspring viability in male Sprague-Dawley rats. Protein synthesis in 2-cell embryos was monitored by [35S] methionine labeling and two-dimensional SDS gel electrophoresis. Fertility was reduced in males with blood lead levels in the range 27-60 microg/dL. Lead was found to affect initial genomic expression in embryos fathered by male rats with blood lead levels as low as 15-23 microg/dL. Dose-dependent increases were seen in an unidentified set of proteins with a relative molecular weight of approximately 70 kDa (Mr). These results indicate that male-mediated effects of lead may be observed in the 2-cell embryo. The alteration observed in embryonic gene expression with paternal lead exposure may be useful for studying the role of the paternal contribution to the activation of the embryonic genome and protein synthesis in the early embryo.
本研究旨在确定相对低水平的醋酸铅（25和250 ppm）暴露对雄性斯普拉格-道利大鼠生育力和后代活力的影响。通过[35S]甲硫氨酸标记和二维SDS凝胶电泳监测2细胞胚胎中的蛋白质合成。血铅水平在27 - 60微克/分升范围内的雄性大鼠生育力降低。发现铅会影响血铅水平低至15 - 23微克/分升的雄性大鼠所产胚胎的初始基因表达。在一组相对分子量约为70 kDa（Mr）的未鉴定蛋白质中观察到剂量依赖性增加。这些结果表明，在2细胞胚胎中可能观察到铅的雄性介导效应。父源性铅暴露导致的胚胎基因表达改变可能有助于研究父源因素在激活胚胎基因组和早期胚胎蛋白质合成中的作用。
