Shibata Y, Yamamoto T
Cell Tissue Res. 1976 Sep 29;172(4):487-501. doi: 10.1007/BF00220334.
Fine structural and cytochemical studies were performed to examine the nature of three types of specific granules found in the atrium of lamprey; specific granules of the atrial muscle cell (ASG), interstitial cell granules (ICG) and endocardial endothelial granules (ESG). Ultrastructurally, ASG and ICG appeared quite similar in size, shape and electron opacity, while ESG were much larger and less dense in opacity than the other two. None of the granules showed positive DAB reaction or acid phosphatase reaction. Only ICG revealed positive chromaffin reaction, which agreed with formaldehyde induced green fluorescence along the atrial lumen. Phosphotungstic acid at low pH stained ICG and ASG strongly positive, and ESG weakly positive. Pronase treatment in Epon sections for 24 h digested ASG alone, whereas in glycol-methacrylate embedded sections, ESG were digested first, ASG were digested thoroughly after 30 min, but ICG were not digested completely after 90 min. From these results it can be concluded that the three types of specific granules have different constituents. ESG consist of protein with some polysaccharides; ASG are composed of protein carbohydrate complexes and lack catecholamines; ICG contain catecholamine as well as protein carbohydrate complexes.
进行了精细结构和细胞化学研究，以检查在七鳃鳗心房中发现的三种特定颗粒的性质；心房肌细胞特异性颗粒（ASG）、间质细胞颗粒（ICG）和心内膜内皮颗粒（ESG）。在超微结构上，ASG和ICG在大小、形状和电子不透明度方面看起来非常相似，而ESG比其他两种颗粒大得多且不透明度较低。这些颗粒均未显示阳性DAB反应或酸性磷酸酶反应。只有ICG显示阳性嗜铬反应，这与沿心房腔的甲醛诱导的绿色荧光一致。低pH值的磷钨酸对ICG和ASG染色呈强阳性，对ESG染色呈弱阳性。在Epon切片中用链霉蛋白酶处理24小时仅消化ASG，而在乙二醇甲基丙烯酸酯包埋的切片中，ESG首先被消化，30分钟后ASG被彻底消化，但90分钟后ICG未被完全消化。从这些结果可以得出结论，这三种特定颗粒具有不同的成分。ESG由含有一些多糖的蛋白质组成；ASG由蛋白质碳水化合物复合物组成且缺乏儿茶酚胺；ICG含有儿茶酚胺以及蛋白质碳水化合物复合物。
