Kobayashi Y
First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1999 Mar;66(1):46-56. doi: 10.5357/koubyou.66.46.
The purpose of this study was to estimate the usefulness of plasma GST-pi in diagnosis of oral SCC and to confirm the expression and localization of GST-pi in human oral SCC. Plasma GST-pi levels of 109 untreated oral SCC cases, 47 recurrent oral SCC cases and 36 healthy controls were measured by the sandwich enzyme-immunoassay (EIA) system. Human oral SCC and human oral SCC cell lines (HSC-3, HSC-4) were examined immunohistochemically and by the immunoelectron microscopic technique. The results were as follows: 1. The cut off level was 30.0 ng/ml (mean+2 S.D.). There was a statistically significant difference between the mean plasma GST-pi levels in oral SCC (both untreated and recurrent cases) and controls (p < 0.001). 2. The positive rate of GST-pi was 63.3% in untreated cases and 68.1% in recurrent cases. 3. There was no significant correlation between plasma GST-pi levels and each clinical factor. 4. The positive rates of plasma GST-pi were compared with those of serum SCC antigen in oral SCC. Plasma GST-pi was 62.9%, while serum SCC antigen 22.2%. 5. From immunohistochemical observation, GST-pi positive staining was evident in the cytoplasm and nucleus of oral SCC. 6. By immunoelectron microscopy, GST-pi was confirmed to be in the mitochondria and the inter-chromatinic regions of the nucleus.
本研究的目的是评估血浆谷胱甘肽S-转移酶π（GST-pi）在口腔鳞状细胞癌（SCC）诊断中的效用，并确认GST-pi在人类口腔SCC中的表达及定位。采用夹心酶免疫分析（EIA）系统检测了109例未经治疗的口腔SCC患者、47例复发性口腔SCC患者及36例健康对照者的血浆GST-pi水平。采用免疫组织化学和免疫电子显微镜技术对人类口腔SCC及人类口腔SCC细胞系（HSC-3、HSC-4）进行了检测。结果如下：1. 临界值为30.0 ng/ml（均值+2标准差）。口腔SCC（未经治疗和复发病例）患者与对照组的血浆GST-pi平均水平存在统计学显著差异（p<0.001）。2. 未经治疗病例中GST-pi的阳性率为63.3%，复发病例中为68.1%。3. 血浆GST-pi水平与各临床因素之间无显著相关性。4. 比较了口腔SCC患者血浆GST-pi与血清鳞状细胞癌抗原的阳性率。血浆GST-pi为62.9%，而血清鳞状细胞癌抗原为22.2%。5. 免疫组织化学观察显示，口腔SCC的细胞质和细胞核中GST-pi呈阳性染色。6. 通过免疫电子显微镜证实，GST-pi存在于线粒体和细胞核的染色质间区域。
