Biesgen C, Weiler E W
Lehrstuhl für Pflanzenphysiologie, Fakultät für Biologie Ruhr-Universität, Bochum, Germany.
Planta. 1999 Apr;208(2):155-65. doi: 10.1007/s004250050545.
The genes of two closely related 12-oxophytodienoic acid reductases (EC 1.3.1.42), OPR1 and OPR2, were identified on a 7079-bp-long genomic fragment from Arabidopsis thaliana (L.) Heynh. The organization of these two genes was determined and putative cis elements were identified. Promoter-beta-glucuronidase (GUS) fusions expressed in transgenic Arabidopsis thaliana and Nicotiana tabacum L. plants revealed differences in OPR-promoter-driven GUS expression in flowers. While the OPR1 promoter directed GUS expression in young seeds, the OPR2 promoter directed pollen-specific expression. Both OPR1 and OPR2, were predominantly expressed in roots. Stress treatments, like local and systemic wounding, UV-C illumination and coldness, resulted in transient changes in steady-state OPR mRNA levels, but no concurrent changes in polypeptide level or enzyme activity were detected.
在来自拟南芥（Arabidopsis thaliana (L.) Heynh.）的一段7079 bp长的基因组片段上，鉴定出了两个密切相关的12-氧代植物二烯酸还原酶（EC 1.3.1.42）的基因，即OPR1和OPR2。确定了这两个基因的结构，并鉴定出了假定的顺式元件。在转基因拟南芥和烟草植株中表达的启动子-β-葡萄糖醛酸酶（GUS）融合体揭示了OPR启动子驱动的GUS在花中的表达差异。虽然OPR1启动子指导GUS在幼嫩种子中表达，但OPR2启动子指导花粉特异性表达。OPR1和OPR2均主要在根中表达。局部和全身创伤、UV-C照射和低温等胁迫处理导致OPR mRNA稳态水平发生短暂变化，但未检测到多肽水平或酶活性的同时变化。
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