You Shuang-Hong, Chen Yuan-Ping, Shi Wen-Jing, Li Xue, Wu Zheng, Yao Quan-Hong
Fruit Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing, China.
Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
Front Plant Sci. 2025 Feb 26;16:1509472. doi: 10.3389/fpls.2025.1509472. eCollection 2025.
12-oxo-phytodienoic acid reductase (OPR) is one of the key enzymes in the octadecanoid pathway, and it controls the last step of jasmonic acid (JA) biosynthesis. Although multiple isoforms and functions of s have been identified in various plants, no genes have been identified, and their possible roles in grapevine development and defense mechanisms remain unknown. In this study, nine genes were identified from grapevine genome and classified into two subfamilies. Systematic analyses of the physical and chemical properties, the expression and structure of the genes, promoter elements, and chromosome locations were performed via bioinformatics and molecular biology methods. In addition, we described the characterization of the gene , which was synthesized via a PCR-based two-step DNA synthesis quantification reverse-transcription (PTDS) method. expression is tissue-specific and induced by various stresses. The overexpression of in and rice (OT) significantly increased tolerance to Cu, Zn stress, and Cu, Zn stress-induced restriction of the germination rate, root/shoot length and fresh weight was significantly alleviated in OT. In OT, enhanced the photosynthetic capacity, promoted ABA synthesis and the ABA-dependent stress response pathway, improved the antioxidation capacity by increasing the activities of ROS scavengers and the expression level of the related genes, while enhancing the accumulation of proline, AsA, GSH and reducing MDA and HO levels. Moreover, reduced Cu, Zn accumulation and translocation. Together, we first systematically characterized the grapevine OPR gene family and reported that responded to Cu, Zn stress in an ABA-dependent manner, and was quite independent of JA synthesis and signaling. All of the above results provide an important research basis and theoretical basis for further revealing the functions of in grapevines in the future.
12-氧代植物二烯酸还原酶（OPR）是十八碳酸途径中的关键酶之一，它控制茉莉酸（JA）生物合成的最后一步。尽管已在多种植物中鉴定出该酶的多种同工型和功能，但尚未鉴定出相关基因，其在葡萄发育和防御机制中的可能作用仍不清楚。在本研究中，从葡萄基因组中鉴定出9个OPR基因，并将其分为两个亚家族。通过生物信息学和分子生物学方法对OPR基因的理化性质、表达、结构、启动子元件和染色体定位进行了系统分析。此外，我们描述了通过基于PCR的两步DNA合成定量逆转录（PTDS）方法合成的VvOPR1基因的特征。VvOPR1的表达具有组织特异性，并受多种胁迫诱导。VvOPR1在拟南芥和水稻（OT）中的过表达显著提高了对铜、锌胁迫的耐受性，并且在OT中显著缓解了铜、锌胁迫诱导的发芽率、根/茎长度和鲜重的限制。在OT中，VvOPR1增强了光合能力，促进了脱落酸（ABA）合成和ABA依赖的胁迫响应途径，通过增加活性氧清除剂的活性和相关基因的表达水平提高了抗氧化能力，并同时增强了脯氨酸、抗坏血酸（AsA）、谷胱甘肽（GSH）的积累，降低了丙二醛（MDA）和过氧化氢（H₂O₂）水平。此外，VvOPR1减少了铜、锌的积累和转运。总之，我们首次系统地表征了葡萄OPR基因家族，并报道VvOPR1以ABA依赖的方式响应铜、锌胁迫，且与JA合成和信号传导相当独立。以上所有结果为未来进一步揭示VvOPR1在葡萄中的功能提供了重要的研究基础和理论依据。
