Walker J, Borrow R, Goering R V, Egerton S, Fox A J, Oppenheim B A
Manchester Public Health Laboratory, Withington Hospital, Manchester.
Creighton University School of Medicine, Omaha, Nebraska 68178, USA.
J Med Microbiol. 1999 Mar;48(3):297-301. doi: 10.1099/00222615-48-3-297.
Bacteriophage typing is currently the recognised methodology for the typing of methicillin-resistant Staphylococcus aureus (MRSA) in the UK. Bacteriophage typing is less discriminatory and does not type all isolates compared with some molecular methods for typing MRSA. Chromosomal genotyping by pulsed-field gel electrophoresis (PFGE) is increasingly recognised as an improved method for typing MRSA, providing increased discrimination and typability. In this study the results of a comparison of bacteriophage typing and PFGE typing and subtyping are presented for a large collection of isolates from the North-West of England. Isolates belonging to the most frequently isolated epidemic methicillin-resistant Staphylococcus aureus (EMRSA) bacteriophage types 15 and 16 were typed by PFGE with further discrimination of common PFGE types possible into a number of subtypes. These results for a large collection of isolates demonstrate the improved typing of MRSA with PFGE. The widespread acceptance of PFGE for typing MRSA isolates has been hampered by the lack of standardised methodologies. Recently, a standardised PFGE strain typing system, known as the GenePath system has become available. The results of an inter-laboratory comparison of PFGE typing for a collection of isolates demonstrated good reproducibility with this system.
噬菌体分型目前是英国认可的耐甲氧西林金黄色葡萄球菌（MRSA）分型方法。与一些MRSA分子分型方法相比，噬菌体分型的鉴别力较低，且不能对所有分离株进行分型。脉冲场凝胶电泳（PFGE）进行的染色体基因分型越来越被认为是一种改进的MRSA分型方法，具有更高的鉴别力和分型能力。在本研究中，展示了对英格兰西北部大量分离株进行噬菌体分型与PFGE分型及亚型分析的比较结果。属于最常分离的流行耐甲氧西林金黄色葡萄球菌（EMRSA）噬菌体类型15和16的分离株通过PFGE分型，常见的PFGE类型可进一步细分为多个亚型。大量分离株的这些结果证明了PFGE对MRSA分型的改进。缺乏标准化方法阻碍了PFGE在MRSA分离株分型中的广泛应用。最近，一种标准化的PFGE菌株分型系统，即GenePath系统已可用。对一组分离株进行PFGE分型的实验室间比较结果表明，该系统具有良好的重复性。
