Nanda N, Iismaa S E, Copeland N G, Gilbert D J, Jenkins N, Graham R M, Sutrave P
Molecular Cardiology Unit, Victor Chang Cardiac Research Institute, 384 Victoria Street, Darlinghurst, New South Wales, 2010, Australia.
Arch Biochem Biophys. 1999 Jun 1;366(1):151-6. doi: 10.1006/abbi.1999.1189.
The mouse Gh/tissue transglutaminase gene (Tgm2), coding a dual-function protein that both binds guanosine triphosphate (GTP) and catalyzes the posttranslational modification of proteins by transamidation of glutamine residues, has been cloned. Sequence analysis of Tgm2 and comparison with the TGase sequences of other species allowed correction of several apparent sequencing artifacts in the Tgm2 cDNA. Tgm2 spans approximately 34 kb and has 13 exons and 12 introns. Although the structure of Tgm2 shows similarity to that of other transglutaminase genes, with introns ranging from 921 bp to >5 kb, several introns differ considerably in size from those of the human Gh gene, TGM2. Tgm2 maps to the distal region of mouse chromosome 2, a region syntenic to human chromosome 20q containing TGM2. Tgm2 is in the vicinity of two uncloned mouse mutations, diminutive (dm) and blind-sterile (bs). Genomic DNA from dm mice was unavailable; however, Southern blot analysis of bs DNA showed no gross rearrangements of Tgm2.
小鼠谷氨酰胺转移酶/组织转谷氨酰胺酶基因（Tgm2）已被克隆，该基因编码一种双功能蛋白，既能结合三磷酸鸟苷（GTP），又能通过谷氨酰胺残基的转酰胺作用催化蛋白质的翻译后修饰。对Tgm2进行序列分析并与其他物种的谷氨酰胺转移酶序列进行比较，校正了Tgm2 cDNA中几个明显的测序假象。Tgm2跨度约为34 kb，有13个外显子和12个内含子。虽然Tgm2的结构与其他转谷氨酰胺酶基因的结构相似，内含子长度从921 bp到大于5 kb不等，但有几个内含子的大小与人类谷氨酰胺转移酶基因TGM2的内含子有很大差异。Tgm2定位于小鼠2号染色体的远端区域，该区域与包含TGM2的人类20号染色体q臂同线。Tgm2位于两个未克隆的小鼠突变体——矮小（dm）和盲不育（bs）附近。无法获得来自dm小鼠的基因组DNA；然而，对bs DNA的Southern印迹分析表明Tgm2没有明显的重排。
