蓖麻油中蓖麻醇酸的生物合成。

Biosynthesis of ricinoleate in castor oil.

作者信息

McKeon T A, Lin J T, Stafford A E

机构信息

Western Regional Research Center, U.S. Dept. of Agriculture, Albany, California 94710, USA.

出版信息

Adv Exp Med Biol. 1999;464:37-47. doi: 10.1007/978-1-4615-4729-7_4.

DOI:10.1007/978-1-4615-4729-7_4

PMID:10335384

Abstract

Castor oil is 90% ricinoleate (12-hydroxyoleate) and has numerous industrial uses. Components of castor bean (Ricinus communis L.) pose serious problems to processors. Other researchers have cloned the gene for the oleoyl hydroxylase, but transgenic plants produce only about 20% hydroxy fatty acid. To improve such transgenic substitutes for castor, we are using HPLC analysis of castor bean microsomal suspensions to follow the hydroxylase reaction and the movement of 14C-ricinoleate through phospholipid into triacylglycerol. Most labeled ricinoleate is rapidly removed from the phospholipid fraction as free fatty acid and incorporated into triacylglycerol, with triricinolein predominating. Elucidation of the basis for high incorporation of ricinoleate and exclusion of oleate from triacylglycerols will identify genes that can be used to engineer high ricinoleate production in transgenic plants.

摘要

蓖麻油90%是蓖麻醇酸酯（12-羟基油酸酯），有多种工业用途。蓖麻子（Ricinus communis L.）的成分给加工者带来严重问题。其他研究人员已克隆了油酰羟化酶基因，但转基因植物仅产生约20%的羟基脂肪酸。为改进此类蓖麻的转基因替代品，我们正在使用高效液相色谱法分析蓖麻子微粒体悬浮液，以追踪羟化酶反应以及14C-蓖麻醇酸酯通过磷脂进入三酰甘油的过程。大多数标记的蓖麻醇酸酯作为游离脂肪酸迅速从磷脂部分去除，并掺入三酰甘油中，其中三蓖麻醇酸甘油酯占主导。阐明蓖麻醇酸酯高掺入和油酸从三酰甘油中排除的基础，将鉴定出可用于在转基因植物中设计高蓖麻醇酸酯产量的基因。