并存胎儿的完全性葡萄胎病例中的DNA流式细胞术定量及DNA多态性分析
DNA flow cytometric quantification and DNA polymorphism analysis in the case of a complete mole with a coexisting fetus.
作者信息
Hirose M, Kimura T, Mitsuno N, Wakuda K, Takakura K, Fujita J, Noda Y
机构信息
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Japan.
出版信息
J Assist Reprod Genet. 1999 May;16(5):263-7. doi: 10.1023/a:1020319629999.
PURPOSE
Our purpose was to investigate whether DNA flow cytometric quantification and DNA polymorphism analysis are useful for cytogenetic diagnosis in the case of a complete hydatidiform mole that coexists with a living fetus.
METHODS
Flow cytometric analysis of the nuclear DNA content and polymerase chain reaction (PCR) amplification of the minisatellite locus with the MCT118 probe were performed on the tissues (fetus, placenta and mole) obtained at the initial evacuation.
RESULTS
DNA histograms of placental, fetal, and molar tissues showed diploid peaks. PCR products demonstrated that the allele of the mole was homozygous and inherited solely from the husband and that the mole differed genetically from the fetus and the placenta.
CONCLUSIONS
These results suggested that DNA flow cytometry and DNA polymorphism analysis may be useful for the cytogenetic diagnosis of a complete hydatidiform mole and a coexisting fetus.
目的
我们的目的是研究在完全性葡萄胎与存活胎儿共存的情况下，DNA流式细胞术定量分析和DNA多态性分析是否有助于细胞遗传学诊断。
方法
对初次清宫时获取的组织（胎儿、胎盘和葡萄胎）进行核DNA含量的流式细胞术分析，并用MCT118探针进行小卫星位点的聚合酶链反应（PCR）扩增。
结果
胎盘、胎儿和葡萄胎组织的DNA直方图显示为二倍体峰。PCR产物表明，葡萄胎的等位基因是纯合的，且仅从丈夫遗传而来，并且葡萄胎在基因上与胎儿和胎盘不同。
结论
这些结果提示，DNA流式细胞术和DNA多态性分析可能有助于完全性葡萄胎及共存胎儿的细胞遗传学诊断。
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