用于真核表达和免疫亲和纯化Gal4融合蛋白的杆状病毒转移载体。 - Suppr

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超能文献

Baculovirus-transfer vector for eukaryotic expression and immunoaffinity purification of Gal4-fusion proteins.

作者信息

Chen G, Courey A J

机构信息

Department of Chemistry and Biochemistry, University of California, Los Angeles 90095-1569, USA.

出版信息

Biotechniques. 1999 May;26(5):808-10, 812, 814. doi: 10.2144/99265bm02.

DOI:10.2144/99265bm02

PMID:10337466

Abstract

摘要

相似文献

Baculovirus-transfer vector for eukaryotic expression and immunoaffinity purification of Gal4-fusion proteins.

Biotechniques. 1999 May;26(5):808-10, 812, 814. doi: 10.2144/99265bm02.

Overproduction and single-step purification of GAL4 fusion proteins from Escherichia coli.

Gene. 1993 Apr 15;126(1):105-7. doi: 10.1016/0378-1119(93)90596-u.

Expression and purification of recombinant proteins using the baculovirus system.

Curr Protoc Mol Biol. 2004 Feb;Chapter 16:Unit 16.11. doi: 10.1002/0471142727.mb1611s65.

A convenient cloning vector containing the GAL4 DNA-binding domain.

Gene. 1992 Sep 1;118(1):143-4. doi: 10.1016/0378-1119(92)90262-n.

A mammalian expression vector for the expression of GAL4 fusion proteins with an epitope tag and histidine tail.

Anal Biochem. 1994 Dec;223(2):291-8. doi: 10.1006/abio.1994.1587.

Avidin is a promising tag for fusion proteins produced in baculovirus-infected insect cells.

Protein Expr Purif. 1999 Oct;17(1):139-45. doi: 10.1006/prep.1999.1123.

A vector, pHisGal, allowing bacterial production of proteins fused to a hexahistidine-tagged Gal4 DNA-binding domain.

Biotechniques. 1994 Oct;17(4):714-6, 718.

A vector for expressing GAL4(1-147) fusions in mammalian cells.

Nucleic Acids Res. 1989 Sep 25;17(18):7539. doi: 10.1093/nar/17.18.7539.

Overview of the baculovirus expression system.

Curr Protoc Mol Biol. 2004 Feb;Chapter 16:Unit 16.9. doi: 10.1002/0471142727.mb1609s65.

A modular set of prokaryotic and eukaryotic expression vectors.

Anal Biochem. 2000 Jan 1;277(1):109-20. doi: 10.1006/abio.1999.4383.

引用本文的文献

The Dorsal Rel homology domain plays an active role in transcriptional regulation.

Mol Cell Biol. 2002 Jul;22(14):5089-99. doi: 10.1128/MCB.22.14.5089-5099.2002.

A functional interaction between the histone deacetylase Rpd3 and the corepressor groucho in Drosophila development.

Genes Dev. 1999 Sep 1;13(17):2218-30. doi: 10.1101/gad.13.17.2218.

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Baculovirus-transfer vector for eukaryotic expression and immunoaffinity purification of Gal4-fusion proteins.

作者信息

Chen G, Courey A J

机构信息

Department of Chemistry and Biochemistry, University of California, Los Angeles 90095-1569, USA.

出版信息

Biotechniques. 1999 May;26(5):808-10, 812, 814. doi: 10.2144/99265bm02.

DOI:10.2144/99265bm02

PMID:10337466

Abstract

摘要

相似文献

Baculovirus-transfer vector for eukaryotic expression and immunoaffinity purification of Gal4-fusion proteins.

Biotechniques. 1999 May;26(5):808-10, 812, 814. doi: 10.2144/99265bm02.

Overproduction and single-step purification of GAL4 fusion proteins from Escherichia coli.

Gene. 1993 Apr 15;126(1):105-7. doi: 10.1016/0378-1119(93)90596-u.

Expression and purification of recombinant proteins using the baculovirus system.

Curr Protoc Mol Biol. 2004 Feb;Chapter 16:Unit 16.11. doi: 10.1002/0471142727.mb1611s65.

A convenient cloning vector containing the GAL4 DNA-binding domain.

Gene. 1992 Sep 1;118(1):143-4. doi: 10.1016/0378-1119(92)90262-n.

A mammalian expression vector for the expression of GAL4 fusion proteins with an epitope tag and histidine tail.

Anal Biochem. 1994 Dec;223(2):291-8. doi: 10.1006/abio.1994.1587.

Avidin is a promising tag for fusion proteins produced in baculovirus-infected insect cells.

Protein Expr Purif. 1999 Oct;17(1):139-45. doi: 10.1006/prep.1999.1123.

A vector, pHisGal, allowing bacterial production of proteins fused to a hexahistidine-tagged Gal4 DNA-binding domain.

Biotechniques. 1994 Oct;17(4):714-6, 718.

A vector for expressing GAL4(1-147) fusions in mammalian cells.

Nucleic Acids Res. 1989 Sep 25;17(18):7539. doi: 10.1093/nar/17.18.7539.

Overview of the baculovirus expression system.

Curr Protoc Mol Biol. 2004 Feb;Chapter 16:Unit 16.9. doi: 10.1002/0471142727.mb1609s65.

A modular set of prokaryotic and eukaryotic expression vectors.

Anal Biochem. 2000 Jan 1;277(1):109-20. doi: 10.1006/abio.1999.4383.

引用本文的文献

The Dorsal Rel homology domain plays an active role in transcriptional regulation.

Mol Cell Biol. 2002 Jul;22(14):5089-99. doi: 10.1128/MCB.22.14.5089-5099.2002.

A functional interaction between the histone deacetylase Rpd3 and the corepressor groucho in Drosophila development.

Genes Dev. 1999 Sep 1;13(17):2218-30. doi: 10.1101/gad.13.17.2218.