利用杆状病毒系统表达和纯化重组蛋白。

Expression and purification of recombinant proteins using the baculovirus system.

作者信息

Murphy Cheryl Isaac, Piwnica-Worms Helen, Grünwald Stefan, Romanow William G, Francis Nicole, Fan Hua-Ying

机构信息

Aquila Biopharmaceuticals, Worcester, Massachusetts, USA.

出版信息

Curr Protoc Mol Biol. 2004 Feb;Chapter 16:Unit 16.11. doi: 10.1002/0471142727.mb1611s65.

DOI:10.1002/0471142727.mb1611s65

PMID:18265341

Abstract

This unit describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production , how to maximize and scale up recombinant protein production, and how to purify recombinant proteins.

摘要

本单元介绍了如何小规模分析感染重组杆状病毒的细胞中的蛋白质表达，以优化蛋白质生产；如何最大化并扩大重组蛋白生产规模；以及如何纯化重组蛋白。