Li H, Malkin R A
Joint Department of Biomedical Engineering, The University of Tennessee-Memphis and The University of Memphis, 38152, USA.
J Electrocardiol. 1999 Apr;32(2):159-66.
Recent studies have shown sustained tachyarrhythmias in guinea pigs. We hypothesized that guinea pigs could be used as a model of ventricular fibrillation, focusing on defibrillation waveform efficacy and the upper limit of vulnerability to fibrillation. In 10 male guinea pigs, an esophageal/apical pacing electrode configuration was used. The electrocardiogram (ECG) and arterial blood pressure were continuously monitored. T-wave and defibrillation shocks were applied transthoracically. A modified up-down protocol was used. After up-down testing was completed, a tachyarrhythmia was induced without electrical termination. All animals died of a sustained tachyarrhythmia. The monophasic DFT50 (the 50% successful defibrillation voltage, 496 +/-176 V) was larger than the biphasic DFT50 (364+/-94 V, P < .005). The upper limit of vulnerability to fibrillation (ULV50) (the 50% successful induction voltage) was correlated with the DFT50 for both monophasic (r = .82, P < .005) and biphasic shocks (r = .88, P < .005). Its low cost and ease of handling may make the guinea pig a preferred model for some fibrillation and defibrillation studies.
最近的研究表明豚鼠存在持续性快速心律失常。我们假设豚鼠可作为室颤模型，重点关注除颤波形效果和颤动易损性上限。在10只雄性豚鼠中，采用食管/心尖起搏电极配置。持续监测心电图（ECG）和动脉血压。经胸施加T波和除颤电击。使用改良的上下法。上下测试完成后，诱发快速心律失常且不进行电终止。所有动物均死于持续性快速心律失常。单相DFT50（50%成功除颤电压，496±176V）大于双相DFT50（364±94V，P<.005）。单相（r=.82，P<.005）和双相电击（r=.88，P<.005）的颤动易损性上限（ULV50）（50%成功诱发电压）均与DFT50相关。其低成本和易于操作可能使豚鼠成为某些颤动和除颤研究的首选模型。
