Tansho S, Abe S, Tansho T, Yamaguchi H
Department of Microbiology and Immunology, University of Teikyo School of Medicine, Tokyo, Japan.
Microbiol Immunol. 1999;43(3):235-40. doi: 10.1111/j.1348-0421.1999.tb02398.x.
The effect of leukocytes on the anti-Candida activity of neutrophils was examined. Murine neutrophils which were purified from casein-induced peritoneal cells inhibited the mycelial growth of Candida albicans. This anti-Candida activity of neutrophils was augmented by the addition of spleen cells prepared from mice pretreated with bacterial lipopolysaccharide 3 hr before, but not from non-treated mice. The population in the spleen cells, which enhanced the anti-Candida activity of neutrophils, was plastic-plate adherent, nylon-fiber columns adherent and anti-Mac-1 antigen-positive. These immunological profiles suggested that the enhancing cells are classified to splenic macrophages. Peritoneal-exudated macrophages from mice treated with lipopolysaccharide also augmented the anti-Candida activity of neutrophils. These results suggest that the anti-Candida activity of neutrophils may be upregulated by activated macrophages.
研究了白细胞对中性粒细胞抗念珠菌活性的影响。从酪蛋白诱导的腹腔细胞中纯化得到的小鼠中性粒细胞可抑制白色念珠菌的菌丝生长。通过添加3小时前用细菌脂多糖预处理的小鼠制备的脾细胞，中性粒细胞的这种抗念珠菌活性增强，但未处理小鼠的脾细胞则无此作用。增强中性粒细胞抗念珠菌活性的脾细胞群体可黏附于塑料板、尼龙纤维柱，且抗Mac-1抗原呈阳性。这些免疫学特征表明，增强细胞可归类为脾巨噬细胞。用脂多糖处理的小鼠腹腔渗出巨噬细胞也增强了中性粒细胞的抗念珠菌活性。这些结果表明，活化的巨噬细胞可能上调中性粒细胞的抗念珠菌活性。
