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对佐治亚州犬群进行犬布鲁氏菌的血清学调查及玻片凝集试验评估。

A serologic survey of a population of Georgia dogs for Brucella canis and an evaluation of the slide agglutination test.

作者信息

Brown J, Blue J L, Wooley R E, Dreesen D W, Carmichael L E

出版信息

J Am Vet Med Assoc. 1976 Dec 1;169(11):1214-6.

PMID:1033929
Abstract

In a serologic survey of stray and pet dog populations of Georgia, serums were screened for Brucella canis antibodies, using the slide agglutination test. If results were positive, B canis antibody titers were determined, using the standard tube agglutination test. The stray dogs had significantly (P less than 0.01) higher titers than did the pet dogs. The reactor rate was 58% higher for the slide agglutination test than for the tube agglutination test. The manufacturer's evaluation of the slide agglutination test was based on a comparison of the serologic results of that test with those of the tube agglutination test, using a comparative method that permitted the results to be interpreted as 99% agreement between the 2 tests. Reevaluation of the manufacturer's data by a different method indicated that the slide agglutination test is very accurate when the results are negative (99.7% specific) but less so when the results are positive (62.5% sensitive).

摘要

在佐治亚州对流浪狗和宠物狗群体进行的一项血清学调查中,使用玻片凝集试验对血清进行犬布鲁氏菌抗体筛查。如果结果呈阳性,则使用标准试管凝集试验测定犬布鲁氏菌抗体滴度。流浪狗的滴度显著高于宠物狗(P小于0.01)。玻片凝集试验的反应率比试管凝集试验高58%。制造商对玻片凝集试验的评估是基于将该试验的血清学结果与试管凝集试验的结果进行比较,采用一种比较方法,该方法可将结果解释为两种试验之间的一致性为99%。用不同方法对制造商的数据进行重新评估表明,玻片凝集试验在结果为阴性时非常准确(特异性为99.7%),但在结果为阳性时准确性较低(敏感性为62.5%)。

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Can Vet J. 2018 Jan;59(1):74-81.
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Brucella canis Isolates from Canadian Dogs.来自加拿大犬类的犬布鲁氏菌分离株。
Can Vet J. 1988 Feb;29(2):149-52.
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A serological survey of dogs for Brucella canis in southwestern Ontario.安大略省西南部犬类布鲁氏菌病血清学调查。
Can Vet J. 1980 Jul;21(7):198-200.
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The sensitivity, specificity and predictive value of total plasma protein in the diagnosis of traumatic reticuloperitonitis.血浆总蛋白在创伤性网胃腹膜炎诊断中的敏感性、特异性及预测价值。
Can J Comp Med. 1983 Jul;47(3):241-4.
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Canine brucellosis: bacteriological and serological investigation of naturally infected dogs in Mexico City.犬布鲁氏菌病:墨西哥城自然感染犬的细菌学和血清学调查
J Clin Microbiol. 1977 Dec;6(6):591-7. doi: 10.1128/jcm.6.6.591-597.1977.